Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. Abstract Text:

Saba Valadkhan,Afshin Mohammadi,Chaim Wachtel,James L Manley,Saba Valadkhan,Afshin Mohammadi,Chaim Wachtel,James L Manley,

Splicing of introns from mRNA precursors is a two-step reaction performed by the spliceosome, an immense cellular machine consisting of over 200 different proteins and five small RNAs (snRNAs). We previously demonstrated that fragments of two of these RNAs, U6 and U2, can catalyze by themselves a splicing-related reaction, involving one of the two substrates of the first step of splicing, the branch site substrate. Here we show that these same RNAs can catalyze a reaction between RNA sequences that resemble the 5' splice site and the branch site, the two reactants of the first step of splicing. The reaction is dependent on the sequence of the 5' splice site consensus sequence and the catalytically essential domains of U6, and thus it resembles the authentic splicing reaction. Our results demonstrate the ability of protein-free snRNAs to recognize the sequences involved in the first splicing step and to perform splicing-related catalysis between these two pre-mRNA-like substrates.

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. Publishing Authors By Initials

S Valadkhan,A Mohammadi,C Wachtel,JL Manley,S Valadkhan,A Mohammadi,C Wachtel,JL Manley,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: RNA (New York, N.Y.)

VOLUME: 13

Page Numbers: 2300-11

Journal Abbreviation: RNA

ISSN: 1469-9001

DAY: 16

MONTH: 10

YEAR: 2007

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509184

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.

AFFILIATION: Center for RNA Molecular Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA. saba.valadkhan@case.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM078572

ACRONYM: GM

MEDLINETA: RNA

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing Related Publications

• A complex signaling pathway regulates SRp38 phosphorylation and pre-mrna splicing in response to heat shock.
• A ported, proximally-cemented femoral stem for total hip arthroplasty. Development and clinical application.
• Aquaporins: role in cerebral edema and brain water balance.
• Atypical memory phenotype T cells with low homeostatic potential and impaired TCR signaling and regulatory T cell function in Foxn1Delta/Delta mutant mice.
• Concurrent splicing and transcription are not sufficient to enhance splicing efficiency.
• Diabetes mellitus in childhood; report of a local survey.
• Dose-response relationship of mannitol and intracranial pressure: a metaanalysis.
• Estimated average glucose derived from HbA(1c) (eAG): report from European Association for the Study of Diabetes (EASD), Amsterdam 2007.
• Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia.
• Human capping enzyme promotes formation of transcriptional R loops in vitro.
• New approaches to physiological informatics in neurocritical care.
• New insights into mitotic chromosome condensation: a role for the prolyl isomerase pin1.
• Pin1 modulates RNA polymerase II activity during the transcription cycle.
• Prehospital hypoxia affects outcome in patients with traumatic brain injury: a prospective multicenter study.
• Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.
• Relationship between brain tissue oxygen tension and CT perfusion: feasibility and initial results.
• Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus.
• Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex.
• Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function.
• T cell development from kit-negative progenitors in the foxn1{delta}/{delta} mutant thymus.
• The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability.
• The impact of smoking-cessation intervention by multiple health professionals.
• The isolation and characterization of glycosaminoglycans in normal human serum.
• The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination.
• The prolyl isomerase Pin1 functions in mitotic chromosome condensation.
• The role of lung function in brain tissue oxygenation following traumatic brain injury.
• The search for alternative splicing regulators: new approaches offer a path to a splicing code.
• Vancomycin use in patients requiring hemodialysis: a literature review.
• Variations in intracellular levels of TATA binding protein can affect specific genes by different mechanisms.
• hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing.