Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases.

Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases. Research Abstract Details 

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Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases. Abstract Text:

Nasreen Sultana,John B Schenkman,James F Rusling,

This communication demonstrates direct electron delivery from electrodes to cyt P450 reductases in stable films ( approximately 100 nm thick) of genetically enriched CYP1A2 and CYP3A4 microsomes made by layer-by-layer assembly with polyions. Reversible voltammetry of films containing genetically enriched cyt P450 monooxygenase microsomes was shown to involve cyt P450 reductase by comparison with the pure rabbit reductase and by lack of characteristic reactions of iron heme enzymes, such as reaction of the FeII form with CO and catalytic electrochemical reduction of oxygen and hydrogen peroxide. The microsome films were activated electrochemically to catalyze styrene epoxidation, consistent with the pathway utilized in the human liver, although further work is required to establish this definitively.

Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases. Publishing Authors By Initials

N Sultana,JB Schenkman,JF Rusling,

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Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 13460-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 5

MONTH: Oct

YEAR: 2005

Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases. Information

Substance Name: Cytochrome P-450 Enzyme System

Registry Number: 9035-51-2

Grant and Affiliation Information for Protein film electrochemistry of microsomes genetically enriched in human cytochrome p450 monooxygenases.

AFFILIATION: Department of Chemistry, University of Connecticut, U-60, Storrs, Connecticut 06269-3060, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: ES03154

ACRONYM: ES

MEDLINETA: J Am Chem Soc

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