Protein design by directed evolution.

Protein design by directed evolution. Research Abstract Details 

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Protein design by directed evolution. Abstract Text:

While nature evolved polypeptides over billions of years, protein design by evolutionary mimicry is progressing at a far more rapid pace. The mutation, selection, and amplification steps of the evolutionary cycle may be imitated in the laboratory using existing proteins, or molecules created de novo from random sequence space, as starting templates. However, the astronomically large number of possible polypeptide sequences remains an obstacle to identifying and isolating functionally interesting variants. Intelligently designed libraries and improved search techniques are consequently important for future advances. In this regard, combining experimental and computational methods holds particular promise for the creation of tailored protein receptors and catalysts for tasks unimagined by nature.

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Protein design by directed evolution. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Annual review of biophysics

VOLUME: 37

Page Numbers: 153-73

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ISSN: 1936-122X

DAY: 9

MONTH: Jun

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 101469708

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Grant and Affiliation Information for Protein design by directed evolution.

AFFILIATION: Laboratory of Organic Chemistry, ETH Zurich, CH-8093 Zurich, Switzerland; email: jaeckel@org.chem.ethz.ch , kast@org.chem.ethz.ch , hilvert@org.chem.ethz.ch.

Country: United States

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MEDLINETA: Annu Rev Biophys

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