Protein-bound acrolein: potential markers for oxidative stress.

Protein-bound acrolein: potential markers for oxidative stress. Research Abstract Details 

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Protein-bound acrolein: potential markers for oxidative stress. Abstract Text:

K Uchida,M Kanematsu,K Sakai,T Matsuda,N Hattori,Y Mizuno,D Suzuki,T Miyata,N Noguchi,E Niki,T Osawa,

Acrolein (CH2==CH---CHO) is known as a ubiquitous pollutant in the environment. Here we show that this notorious aldehyde is not just a pollutant, but also a lipid peroxidation product that could be ubiquitously generated in biological systems. Upon incubation with BSA, acrolein was rapidly incorporated into the protein and generated the protein-linked carbonyl derivative, a putative marker of oxidatively modified proteins under oxidative stress. To verify the presence of protein-bound acrolein in vivo, the mAb (mAb5F6) against the acrolein-modified keyhole limpet hemocyanin was raised. It was found that the acrolein-lysine adduct, Nepsilon-(3-formyl-3, 4-dehydropiperidino)lysine, constitutes an epitope of the antibody. Immunohistochemical analysis of atherosclerotic lesions from a human aorta demonstrated that antigenic materials recognized by mAb5F6 indeed constituted the lesions, in which intense positivity was associated primarily with macrophage-derived foam cells and the thickening neointima of arterial walls. The observations that (i) oxidative modification of low-density lipoprotein with Cu2+ generated the acrolein-low-density lipoprotein adducts and (ii) the iron-catalyzed oxidation of arachidonate in the presence of protein resulted in the formation of antigenic materials suggested that polyunsaturated fatty acids are sources of acrolein that cause the production of protein-bound acrolein. These data suggest that the protein-bound acrolein represents potential markers of oxidative stress and long-term damage to protein in aging, atherosclerosis, and diabetes.

Protein-bound acrolein: potential markers for oxidative stress. Publishing Authors By Initials

K Uchida,M Kanematsu,K Sakai,T Matsuda,N Hattori,Y Mizuno,D Suzuki,T Miyata,N Noguchi,E Niki,T Osawa,

For similar proteins: albumins: serum albumin: serum albumin, bovine research abstracts see: proteins: albumins: serum albumin: serum albumin, bovine research

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Protein-bound acrolein: potential markers for oxidative stress. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 95

Page Numbers: 4882-7

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 28

MONTH: Apr

YEAR: 1998

Protein-bound acrolein: potential markers for oxidative stress. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Protein-bound acrolein: potential markers for oxidative stress. Keywords Mesh Terms:

KEYWORDS: Serum Albumin, Bovine

MESH TERMS: immunology

Chemical & Substance for Abstract: Protein-bound acrolein: potential markers for oxidative stress. Information

Substance Name: Histidine

Registry Number: 71-00-1

Grant and Affiliation Information for Protein-bound acrolein: potential markers for oxidative stress.

AFFILIATION: Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan. uchidak@agr.nagoya-u.ac.jp

Country: UNITED STATES

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MEDLINETA: Proc Natl Acad Sci U S A

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