Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase.

Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Research Abstract Details 

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Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Abstract Text:

Kazunori Watanabe,Yukimatsu Toh,Kyoko Suto,Yoshihiro Shimizu,Natsuhisa Oka,Takeshi Wada,Kozo Tomita,

Eubacterial leucyl/phenylalanyl-tRNA protein transferase (LF-transferase) catalyses peptide-bond formation by using Leu-tRNA(Leu) (or Phe-tRNA(Phe)) and an amino-terminal Arg (or Lys) of a protein, as donor and acceptor substrates, respectively. However, the catalytic mechanism of peptide-bond formation by LF-transferase remained obscure. Here we determine the structures of complexes of LF-transferase and phenylalanyl adenosine, with and without a short peptide bearing an N-terminal Arg. Combining the two separate structures into one structure as well as mutation studies reveal the mechanism for peptide-bond formation by LF-transferase. The electron relay from Asp 186 to Gln 188 helps Gln 188 to attract a proton from the alpha-amino group of the N-terminal Arg of the acceptor peptide. This generates the attacking nucleophile for the carbonyl carbon of the aminoacyl bond of the aminoacyl-tRNA, thus facilitating peptide-bond formation. The protein-based mechanism for peptide-bond formation by LF-transferase is similar to the reverse reaction of the acylation step observed in the peptide hydrolysis reaction by serine proteases.

Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Publishing Authors By Initials

K Watanabe,Y Toh,K Suto,Y Shimizu,N Oka,T Wada,K Tomita,

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Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature

VOLUME: 449

Page Numbers: 867-71

Journal Abbreviation: Nature

ISSN: 1476-4687

DAY: 23

MONTH: 09

YEAR: 2007

Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase.

AFFILIATION: Institute of Biological Resources and Functions, National Institute of Advanced Industrial Sciences and Technology, 1-1-1, Higashi, Tsukuba-shi, Ibaraki 305-8566, Japan.

Country: England

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MEDLINETA: Nature

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ACCESSION NUMBER: 2Z3P

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