[Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum]

[Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum] Research Abstract Details 

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[Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum] Abstract Text:

An-Geng Hu,Yue-Sheng Huang,

OBJECTIVE: To investigate the protective effects of c-jun antisense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum. METHODS: Cardiomyocytes from Wistar rat were isolated and cultured before being divided into normal control (C), transfection (T) and non-transfection (NT) groups. C-jun antisense gene recombinant was constructed and transfected into cardiomyocytes, which were then treated by hypoxia and burn serum in T group, while those in NT group were simply treated by hypoxia and burn serum. The changes in the c-jun mRNA expression were determined by RT-PCR at 1, 3 and 7 hours after the cardiomyocytes being stimulated, and the changes in the expressions of c-jun protein, troponin-T (TnT) and beta-tubulin were assayed by Western blot. The morphological changes in the cardiomyocytes were observed by LM and EM. RESULTS: 1) The expressions of c-jun mRNA and protein in the NT group were increased evidently when compared with those in C and T groups. 2) The expressions of TnT and beta-tubulin in NT group were decreased evidently in contrast to those in C and T groups. In addition, there exhibited evident structural derangement, dissolution and fragmentation to granules of cardiomyocytes in NT group, while the myocardial cytoskeletal structure was well preserved with scarce fragmentation and dissolution in T group. CONCLUSION: Increased expression of c-jun in rat cardiomyocytes resulting in myocardial injury could be induced by combined treatment of hypoxia and burn serum, while c-jun antisense gene recombinant transfection might protect rat cardiomyocytes from injury.

[Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum] Publishing Authors By Initials

AG Hu,YS Huang,

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[Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua shao shang za zhi = Zhonghua shaoshang za

VOLUME: 19

Page Numbers: 267-70

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ISSN: 1009-2587

DAY: 22

MONTH: Oct

YEAR: 2003

[Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum] Information

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LANGUAGE: chi

NlmUniqueID: 100959418

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AFFILIATION: Institute of Burn Research, Southwestern Hospital, State Key Laoratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China.

Country: China

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MEDLINETA: Zhonghua Shao Shang Za Zhi

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