[Protective effect of protein kinase C on heart function: an experiment with isolated rabbit hearts]

[Protective effect of protein kinase C on heart function: an experiment with isolated rabbit hearts] Research Abstract Details 

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[Protective effect of protein kinase C on heart function: an experiment with isolated rabbit hearts] Abstract Text:

Xin Zhang,Ru-kun Chen,Jian-li Niu,

OBJECTIVE: To investigate the protective effect of protein kinase C on heart function. METHODS: The hearts of 40 rabbits were isolated, underwent Langendroff perfusion, and randomly divided into 4 equal groups: Group I (control group, the heart underwent long ischemia or preservation for I hour, was re-warmed and re-infused, and then re-perfused with K-H fluid), Group II (ischemic preconditioning group, perfusion was stopped for 5 minutes before the long ischemia, then the aorta was re-infused), Group III (specific activator of PKC, phorbol myritate acetate was infused inversely via the aorta before the perfusion of K-H fluid), and Group IV (polymyxin B, a specific antagonist of PKC, was infused after the infusion of PKC). Before the preservation and by the end of reperfusion left ventricle end systolic pressure (LVESP), and left ventricle end diastolic pressure (LVDSP) were measured. At the end of experiment specimens of myocardium were collected from each heart to measure the water content, the levels of lactic dehydrogenase and MB isoenzyme of creatine kinase (CK-MB), superoxide dismutate (SOD), and malonyldialdehyde (MDA). TUNEL method was used to measure the number of apoptotic cardiomyocyte. RESULTS: All heart resumed beating. The LVESP of Group II and III were both significantly higher than those of Groups I and IV (all P < 0.05) and the LVESP of Group III was significantly higher than that of Group II too (P < 0.05). The LVEDP of Group II and III were both significantly lower than those of Groups I and IV (all P < 0.05) and the LVEDP of Group III was significantly lower than that of Group II too (P < 0.05). The levels of CK-MB, LDH, and SOD of Group II and III were all significantly higher than those of Groups I and IV (all P < 0.05) and the levels of CK-MB and LDH of Group III was significantly higher than that of Group II too (P < 0.05). The level of MDA of Group II and III were both significantly lower than those of Groups I and IV (all P < 0.05) and the level of MDA of Group III was significantly lower than that of Group II too (P < 0.05). The water contents of Group II and III were both significantly lower than those of Groups I and IV (all P < 0.05). The numbers of TUNEL positive cell and apoptotic cells of Group II and III were all significantly lower than those of Groups I and IV (all P < 0.05). CONCLUSIONS: PKC can be activated by transient ischemia and PMA. PKC protects the heart function effectively.

[Protective effect of protein kinase C on heart function: an experiment with isolated rabbit hearts] Publishing Authors By Initials

X Zhang,RK Chen,JL Niu,

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[Protective effect of protein kinase C on heart function: an experiment with isolated rabbit hearts] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua yi xue za zhi

VOLUME: 86

Page Numbers: 1122-4

Journal Abbreviation: Zhonghua Yi Xue Za Zhi

ISSN: 0376-2491

DAY: 25

MONTH: Apr

YEAR: 2006

[Protective effect of protein kinase C on heart function: an experiment with isolated rabbit hearts] Information

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LANGUAGE: chi

NlmUniqueID: 7511141

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AFFILIATION: Department of Thoracic Surgery, Second Hospital Affiliated to Zhejiang University College of Medicine, Hangzhou 310031, China.

Country: China

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MEDLINETA: Zhonghua Yi Xue Za Zhi

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