Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model.

Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model. Abstract Text:

Jiafen Hu,Nancy M Cladel,Lynn R Budgeon,Cynthia A Reed,Martin D Pickel,Neil D Christensen,

Papillomavirus major capsid protein L1 has successfully stimulated protective immunity against virus infection by induction of neutralizing antibodies in animal models and in clinical trials. However, the potential impact of L1-induced protective cell-mediated immune (CMI) responses is difficult to measure in vivo because of the coincidence of anti-L1 antibody. In this study, we tested the hypothesis that L1 could activate CMI, using the Cottontail Rabbit Papillomavirus (CRPV)-rabbit model. A unique property of this model is that infections can be initiated with viral DNA, thus bypassing all contributions to protection via neutralizing anti-L1 antibody. DNA vaccines containing either CRPV L1, or subfragments of L1 (amino-terminal two-thirds of L1 [L1N] and the carboxylterminal two-thirds of L1 [L1C]), were delivered intracutaneously into rabbits, using a gene gun. After three booster immunizations, the rabbits were challenged with several viral DNA constructs: wild-type CRPV, CRPV L1ATGko (an L1 ATG knockout mutation), and CRPV-ROPV hybrid (CRPV with a replacement L1 from Rabbit Oral Papillomavirus). Challenge of L1 DNA-vaccinated rabbits with wild-type CRPV resulted in significantly fewer papillomas when compared with challenge with CRPV L1ATGko DNA. Significantly smaller papillomas were found in CRPV L1-, L1N-, and L1C-vaccinated rabbits. In addition, rabbits vaccinated with either L1 or L1N grew significantly fewer and smaller papillomas when challenged with CRPV-ROPV hybrid DNA. Therefore, CRPV L1 DNA vaccination induced CMI responses to CRPV DNA infections that can contribute to protective immunity. Cross-protective immunity against CRPV L1 and ROPV L1 was elicited in these CRPV L1- and subfragment-vaccinated rabbits.

Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model. Publishing Authors By Initials

J Hu,NM Cladel,LR Budgeon,CA Reed,MD Pickel,ND Christensen,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Viral immunology

VOLUME: 19

Page Numbers: 492-507

Journal Abbreviation:

ISSN: 0882-8245

DAY: 3

MONTH: 12

YEAR: 2006

Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8801552

Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model.

AFFILIATION: Department of Pathology, Jake Gittlen Cancer Research Foundation, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Viral Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model Related Publications

• A preclinical review of sunitinib, a multitargeted receptor tyrosine kinase inhibitor with anti-angiogenic and antitumour activities.
• An evaluation of tear film breakup time extension and ocular protection index scores among three marketed lubricant eye drops.
• Beta 1, 3-glucan recognition protein from the mosquito, Armigeres subalbatus, is involved in the recognition of distinct types of bacteria in innate immune responses.
• Binding and neutralization efficiencies of monoclonal antibodies, Fab fragments, and scFv specific for L1 epitopes on the capsid of infectious HPV particles.
• Candidate gene polymorphisms in the serotonergic pathway: influence on depression symptomatology in an elderly population.
• Changing the course of Alzheimer's disease: anti-amyloid disease-modifying treatments on the horizon.
• Danish stable schools for experiential common learning in groups of organic dairy farmers.
• Demand-withdraw communication in severely distressed, moderately distressed, and nondistressed couples: rigidity and polarity during relationship and personal problem discussions.
• Design and analysis in genetic studies of human ageing and longevity.
• Effects on the metatarsophalangeal joint after simulated first tarsometatarsal joint arthrodesis.
• Establishment of a cottontail rabbit papillomavirus/HLA-A2.1 transgenic rabbit model.
• Ethics in the dental profession-2006.
• Expression profile of ovarian tumors: distinct signature of Sertoli-Leydig cell tumor.
• Granulicatella elegans bacteraemia in patients with abdominal infections.
• Impact of genetic changes to the CRPV genome and their application to the study of pathogenesis in vivo.
• Increased immunity to cottontail rabbit papillomavirus infection in EIII/JC inbred rabbits after vaccination with a mutant E6 that correlates with spontaneous regression.
• Inference for case-control studies when exposure status is both informatively missing and misclassified.
• Making scents out of spatial and temporal codes in specialist and generalist olfactory networks.
• Necrotizing fasciitis in the head and neck region: an analysis of standard treatment effectiveness.
• Overview of structure and function of mammalian cilia.
• Papillomavirus particles assembled in 293TT cells are infectious in vivo.
• Plasma concentrations of megestrol acetate and medroxyprogesterone acetate after single oral administration to healthy subjects.
• Protective cell-mediated immunity by DNA vaccination against Papillomavirus L1 capsid protein in the Cottontail Rabbit Papillomavirus model.
• Relationship violence among couples seeking therapy: common couple violence or battering?
• Replication of flock house virus in three genera of medically important insects.
• Social activity and healthy aging: a study of aging Danish twins.
• Socioeconomic position and twins' health: a life-course analysis of 1266 pairs of middle-aged Danish twins.
• The potencies of thapsigargin and analogues as activators of rat peritoneal mast cells.
• Transitive inverse-consistent manifold registration.
• Unchanged acetylation of isoniazid by alcohol intake.