Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection.

Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection. Research Abstract Details 

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Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection. Abstract Text:

James D Brien,Jennifer L Uhrlaub,Janko Nikolich-Zugich,

West Nile virus (WNV) is a small, positive-strand RNA virus belonging to the Flaviviridae genus, which causes lethal encephalitis in a subset of infected birds and mammals. In humans, WNV exhibits pronounced age-related morbidity and mortality, but the basis of this effect is unclear, and the molecular and cellular parameters of the host-WNV infection are just beginning to be elucidated. Indeed, numerous mechanisms were implicated in protection in vivo against WNV (IFN-I and IFN-gamma, antibody, C', CD8 and CD4 T cells), but the individual importance of each one of these remains unclear. Here, we show that transfer of highly enriched naïve CD8(+ )T cells protects the majority of alymphoid mice against lethal WNV infection. To substantiate and expand this finding, we defined the peptide specificity of the CD8 response in H-2b mice and used a panel of identified peptides to map one dominant (NS4b (2248-2256)) and several subdominant epitopes. The hierarchy of these epitopes was stably maintained in the memory responses. Most importantly, CTL lines directed against these peptides conferred protection against lethal WNV infection in direct proportion to the epitope immunodominance. These results provide a springboard for future characterization of T cell responses against WNV and demonstrate, for the first time, that CD8 T cells can single-handedly protect from this disease.

Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection. Publishing Authors By Initials

JD Brien,JL Uhrlaub,J Nikolich-Zugich,

For similar virus diseases: arbovirus infections: encephalitis, arbovirus: west nile fever research abstracts see: virus diseases: arbovirus infections: encephalitis, arbovirus: west nile fever research

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Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: European journal of immunology

VOLUME: 37

Page Numbers: 1855-63

Journal Abbreviation: Eur. J. Immunol.

ISSN: 0014-2980

DAY: 3

MONTH: Jul

YEAR: 2007

Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1273201

Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection. Keywords Mesh Terms:

KEYWORDS: West Nile Fever

MESH TERMS: prevention & control

Chemical & Substance for Abstract: Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection. Information

Substance Name: Immunodominant Epitopes

Registry Number: 0

Grant and Affiliation Information for Protective capacity and epitope specificity of CD8(+) T cells responding to lethal West Nile virus infection.

AFFILIATION: Vaccine and Gene Therapy Institute and the Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USA.

Country: Germany

AGENCY: United States NIAID

GRANT: T32 AI007472

ACRONYM: AI

MEDLINETA: Eur J Immunol

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