[Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection]

[Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection] Research Abstract Details 

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[Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection] Abstract Text:

Xiao-lu Li,Yi-zhi Peng,Zhi-qiang Yuan,Yue-sheng Huang,Zong-cheng Yang,

OBJECTIVE: To investigate the protecting effect on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through injury by transfection of recombinant adenovirus with whole long human heat shock protein 70 (HSP70) gene. METHODS: The recombinant adenoviruses were constructed with whole long human HSP70 gene. Cultured intestinal epithelial cells (IEC-6 cells) were divided into four groups: control group without transfection and 24 hours, 48 hours, 72 hours after transfection groups. The expression of human HSP70 gene in transfected cells were detected by reverse transcription-polymerase chain reaction (RT-PCR) after 48 hours transfection. Cells in different groups after 1 hour were treated with hypoxia followed by 1 hour of reoxygenation, then cell viability, growth curve and proliferative index were evaluated in vitro. RESULTS: The expression of human HSP70 gene in transfected IEC-6 cells was positive, while it was negative in control group. After treatment with hypoxia-reoxygenation, the cell viability in transfected groups was significantly higher than that of control (P<0.01), the proliferative index was also improved (P<0.05). CONCLUSION: The proliferation ability of intestinal epithelial cells (IEC-6 cells) could be promoted by HSP70 gene transfection, which therefore could protect the IEC-6 cells against hypoxia-reoxygenation injury.

[Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection] Publishing Authors By Initials

XL Li,YZ Peng,ZQ Yuan,YS Huang,ZC Yang,

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[Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese cr

VOLUME: 15

Page Numbers: 81-3

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ISSN: 1003-0603

DAY: 14

MONTH: Feb

YEAR: 2003

[Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection] Information

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LANGUAGE: chi

NlmUniqueID: 9887521

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Grant and Affiliation Information for [Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection]

AFFILIATION: Institute of Burn Research, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.

Country: China

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MEDLINETA: Zhongguo Wei Zhong Bing Ji Jiu

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