Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors.

Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors. Research Abstract Details 

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Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors. Abstract Text:

Yves Mechulam,Laurent Guillon,Laure Yatime,Sylvain Blanquet,Emmanuelle Schmitt,Yves Mechulam,Laurent Guillon,Laure Yatime,Sylvain Blanquet,Emmanuelle Schmitt,

To decipher the mechanisms of translation initiation, the stability of the complexes between tRNA and initiation factors has to be evaluated in a routine manner. A convenient method to measure the parameters of binding of an aminoacyl-tRNA to an initiation factor results from the property that, when specifically complexed to a protein, the aminoacyl-tRNA often resists spontaneous deacylation. This chapter describes the preparation of suitable aminoacyl-tRNA ligands and their use in evaluating the stability of their complexes with various initiation factors, such as e/aIF2 and e/aIF5B. The advantages and the limitations of the method are discussed.

Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors. Publishing Authors By Initials

Y Mechulam,L Guillon,L Yatime,S Blanquet,E Schmitt,Y Mechulam,L Guillon,L Yatime,S Blanquet,E Schmitt,

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Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in enzymology

VOLUME: 430

Page Numbers: 265-81

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 4

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 212271

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AFFILIATION: Laboratoire de Biochimie, CNRS Ecole Polytechnique, Palaiseau Cedex, France.

Country: United States

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MEDLINETA: Methods Enzymol

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