Prophenoloxidases 1 and 2 from the spruce budworm, Choristoneura fumiferana: Molecular cloning and assessment of transcriptional regulation by a polydnavirus.

Prophenoloxidases 1 and 2 from the spruce budworm, Choristoneura fumiferana: Molecular cloning and assessment of transcriptional regulation by a polydnavirus. Research Abstract Details 

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Prophenoloxidases 1 and 2 from the spruce budworm, Choristoneura fumiferana: Molecular cloning and assessment of transcriptional regulation by a polydnavirus. Abstract Text:

Daniel Doucet,Catherine ,Ashley Dowling,Jocelyne Simard,Qili Feng,Peter J Krell,Michel Cusson,

Immune challenge in arthropods is frequently accompanied by melanization of the hemolymph, a reaction triggered by the activation of prophenoloxidase (PPO). Because their immature stages are spent inside the hemocoel of insect larvae, endoparasitoids have evolved strategies to escape or counter melanin formation. Very little molecular information is available on these endoparasitoid counterstrategies. We have sought to shed light on the inhibition of melanization in the spruce budworm, Choristoneura fumiferana, by the parasitic wasp Tranosema rostrale, by cloning two host PPO homologs and studying their transcriptional regulation after parasitization. The two polypeptides are encoded by transcripts of 3.3 kb (for CfPPO1) and 3.0 kb (for CfPPO2) and possess structural features typical of other insect PPOs. While there appears to be a single CfPPO2 gene in the C. fumiferana genome, we detected three CfPPO1 mRNA variants displaying insertions/deletions in the 3' untranslated region, suggesting that there may be more than one CfPPO1 gene copy. Both CfPPO1 and CfPPO2 were expressed at high levels in C. fumiferana 6th instars, and parasitization by T. rostrale had no apparent impact on the level of their transcripts. Injection of a large dose (0.5 female-equivalent) of polydnavirus-laden calyx fluid extracted from T. rostrale, which is known to inhibit melanization in C. fumiferana, only caused a transient decrease in CfPPO1 and CfPPO2 transcript accumulation at 2-3 d post injection. It thus appears that transcriptional downregulation of C. fumiferana PPO by T. rostrale plays a minor role in the inhibition of hemolymph melanization in this host-parasitoid system. Arch. Insect Biochem. Physiol. 67:188-201, 2008. Published 2008 Wiley-Liss, Inc.(dagger).

Prophenoloxidases 1 and 2 from the spruce budworm, Choristoneura fumiferana: Molecular cloning and assessment of transcriptional regulation by a polydnavirus. Publishing Authors By Initials

D Doucet,C ,A Dowling,J Simard,Q Feng,PJ Krell,M Cusson,

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Prophenoloxidases 1 and 2 from the spruce budworm, Choristoneura fumiferana: Molecular cloning and assessment of transcriptional regulation by a polydnavirus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Archives of insect biochemistry and physiology

VOLUME: 67

Page Numbers: 188-201

Journal Abbreviation: Arch. Insect Biochem. Physiol.

ISSN: 0739-4462

DAY: 24

MONTH: Apr

YEAR: 2008

Prophenoloxidases 1 and 2 from the spruce budworm, Choristoneura fumiferana: Molecular cloning and assessment of transcriptional regulation by a polydnavirus. Information

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LANGUAGE: eng

NlmUniqueID: 8501752

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AFFILIATION: Laurentian Forestry Centre, Natural Resources Canada, Quebec, Canada.

Country: United States

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MEDLINETA: Arch Insect Biochem Physiol

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