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Properties of calcium-dependent regulatory proteins from fungi and yeast.

Properties of calcium-dependent regulatory proteins from fungi and yeast. Research Abstract Details 

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  • Properties of calcium-dependent regulatory proteins from fungi and yeast. Abstract Text:

    t nakamuraT Nakamura,k fujitaK Fujita,y eguchiY Eguchi,m yazawaM Yazawa,

    Calmodulins were isolated from vegetative mycelia of Basidiomycetes fungi, Agaricus campestris and Coprinus lagopus. These calmodulins showed similar mobilities to those of animal calmodulins on nondenaturing polyacrylamide gel electrophoresis in the presence or absence of Ca2+. The molecular weights of both calmodulins were determined to be 16,000. Agaricus calmodulin consisted of 148 amino acids including epsilon-N-trimethyllysine and cysteine. The UV-absorption spectrum showed the relatively high content of phenylalanine in Basidiomycetes calmodulins. The difference UV-absorption spectrum due to the blue shift by Ca2+ was observed. Both calmodulins activated muscle myosin light chain kinase and pea NAD+ kinase in a Ca2+-dependent manner, and the activities were inhibited by trifluoperazine or chlorpromazine. A calmodulin-like protein was partially purified from baker's yeast, Saccharomyces cerevisiae. However, detection of a calmodulin-like protein in prokaryotes was not successful.

    Properties of calcium-dependent regulatory proteins from fungi and yeast. Publishing Authors By Initials

    t nakamuraT Nakamura,k fujitaK Fujita,y eguchiY Eguchi,m yazawaM Yazawa,

    For similar investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research abstracts see: investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research

    PUBMED ID PMID:

    MEDLINE DATE:

    Properties of calcium-dependent regulatory proteins from fungi and yeast. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 95

    Page Numbers: 1551-7

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Jun

    YEAR: 1984

    Properties of calcium-dependent regulatory proteins from fungi and yeast. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Properties of calcium-dependent regulatory proteins from fungi and yeast. Keywords Mesh Terms:

    KEYWORDS: Spectrophotometry, Ultraviolet

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Properties of calcium-dependent regulatory proteins from fungi and yeast. Information

    Substance Name: NAD kinase

    Registry Number: EC 2.7.1.23

    Grant and Affiliation Information for Properties of calcium-dependent regulatory proteins from fungi and yeast.

    AFFILIATION:

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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