Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria.

Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria. Research Abstract Details 

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Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria. Abstract Text:

T Hashimoto,Y Negawa,K Tagawa,

High pressure column chromatography was applied to estimate the bound nucleotides on F1-ATPase. In this way, various species of adenine nucleotide in 0.1 to 0.3 mg of enzyme protein were well separated and estimated with a precision of about 10 nmol within 10 min. ATPase in submitochondrial particles contained 2 mol of ATP and 1 to 2 mol of ADP. The nucleotide content and binding nature of the enzyme varied at different stages of purification, but a total of four binding sites were found in enzyme preparations at all steps. Highly purified enzyme had two tight binding sites for ATP and two loose binding sites, one for ATP and one for ADP. The tight binding of ADP observed in submitochondrial particles was reconstituted by continuous supply of ATP and Mg2+ to the purified enzyme. Removal and rebinding of the nucleotides did not affect ATP-hydrolyzing activity but caused conformational changes of the enzyme, as demonstrated by measuring cold-lability and trypsin digestion. An analog of ATP, AMPP(NH)P, was found to bind to loose binding sites of the purified enzyme with 2 mol of tightly bound ATP, and to inhibit ATP-hydrolyzing activity competitively. The analog also bound to the tight sites under special conditions, protecting the enzyme against cold inactivation. During enzymatic hydrolysis of [3H]ATP, labeled ATP and ADP were both bound at the loose sites, but only slight amounts of these nucleotides were bound to the tight sites. From these results it is inferred that the loose sites are catalytic, while the tight sites are not.

Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria. Publishing Authors By Initials

T Hashimoto,Y Negawa,K Tagawa,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research

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Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 90

Page Numbers: 1141-50

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1981

Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria. Keywords Mesh Terms:

KEYWORDS: Structure-Activity Relationship

MESH TERMS: enzymology

Chemical & Substance for Abstract: Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria. Information

Substance Name: Proton-Translocating ATPases

Registry Number: EC 3.6.3.14

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Country: JAPAN

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MEDLINETA: J Biochem

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