Promotion of tubulin assembly by poorly soluble taxol analogs.

Promotion of tubulin assembly by poorly soluble taxol analogs. Research Abstract Details 

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Promotion of tubulin assembly by poorly soluble taxol analogs. Abstract Text:

Shubhada Sharma,Thota Ganesh,David G I Kingston,Susan Bane,

Promotion or inhibition of tubulin assembly into microtubules is the standard in vitro assay for evaluating potential antimicrotubule agents. Many agents to be tested are poorly soluble in aqueous solution and require a cosolvent such as dimethyl sulfoxide (DMSO). However, DMSO itself can promote tubulin assembly, and its inclusion in assays for compounds that induce tubulin assembly complicates interpretation of the results. Substituting GDP for GTP in the exchangeable nucleotide binding site of tubulin produces a less active form of the protein, tubulin-GDP. Here it is shown that tubulin-GDP can be assembled into normal microtubules in DMSO concentrations up to 15% (v/v), and polymerization assays performed under these conditions can be compared with assays run under more standard conditions. Assays for measuring the effective concentration of a ligand for promotion of tubulin assembly (EC(50)), measuring the concentration for inhibition of tubulin assembly (IC(50)) by a colchicine site ligand, and measuring tubulin critical concentrations in the presence of poorly soluble taxol derivatives are illustrated.

Promotion of tubulin assembly by poorly soluble taxol analogs. Publishing Authors By Initials

S Sharma,T Ganesh,DG Kingston,S Bane,

For similar macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research abstracts see: macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research

PUBMED ID PMID:

MEDLINE DATE:

Promotion of tubulin assembly by poorly soluble taxol analogs. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical biochemistry

VOLUME: 360

Page Numbers: 56-62

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 30

MONTH: 10

YEAR: 2006

Promotion of tubulin assembly by poorly soluble taxol analogs. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

Promotion of tubulin assembly by poorly soluble taxol analogs. Keywords Mesh Terms:

KEYWORDS: Tubulin

MESH TERMS: chemistry

Chemical & Substance for Abstract: Promotion of tubulin assembly by poorly soluble taxol analogs. Information

Substance Name: Guanosine Triphosphate

Registry Number: 86-01-1

Grant and Affiliation Information for Promotion of tubulin assembly by poorly soluble taxol analogs.

AFFILIATION: Department of Chemistry, State University of New York, Binghamton, NY 13902, USA.

Country: United States

AGENCY: United States NCI

GRANT: R01 CA069571-11

ACRONYM: CA

MEDLINETA: Anal Biochem

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