Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells.

Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells. Research Abstract Details 

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Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells. Abstract Text:

Chaur-Jong Hu,Shang-Der Chen,Ding-I Yang,Teng-Nan Lin,Chuan-Mu Chen,Tim Hui-Ming Huang,Chung Y Hsu,

Angiogenesis is induced in response to ischemia. Thrombospondin-1 (TSP-1) is a potent angiostatic factor. Silencing of TSP-1 expression may contribute to the postischemic angiogenesis. Upregulation of TSP-1, in contrast, may terminate the postischemic angiogenesis. A possible mechanism that silences TSP-1 expression is the DNA methylation of its promoter region. DNA methylation has been reported following cerebral ischemia. The present study aimed to explore whether methylation of the promoter region of TSP-1 regulates its expression after oxygen-glucose deprivation (OGD) in murine cerebral endothelial cells (CECs) in vitro. Sublethal OGD increased the extent of methylation of the promoter region of TSP-1 with a concurrent decrease in TSP-1 mRNA and protein expression in CECs. After reoxygenation, demethylation of the TSP-1 promoter region led to the restoration of TSP-1 mRNA and protein expression. The extent of methylation of the promoter region of TSP-1 was inversely correlated with the extent of TSP-1 gene expression at mRNA and protein levels after OGD. Oxygen-glucose deprivation-induced reduction in the TSP-1 mRNA level was not accompanied by a change in mRNA stability. These findings raise the possibility that OGD downregulation of TSP-1 expression is at least in part due to methylation of its promoter region.

Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells. Publishing Authors By Initials

CJ Hu,SD Chen,DI Yang,TN Lin,CM Chen,TH Huang,CY Hsu,

For similar membrane glycoproteins: thrombospondins: thrombospondin 1 research abstracts see: membrane glycoproteins: thrombospondins: thrombospondin 1 research

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Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cerebral blood flow and metabolism : of

VOLUME: 26

Page Numbers: 1519-26

Journal Abbreviation: J. Cereb. Blood Flow Metab.

ISSN: 0271-678X

DAY: 29

MONTH: 03

YEAR: 2006

Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8112566

Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells. Keywords Mesh Terms:

KEYWORDS: Thrombospondin 1

MESH TERMS: genetics

Chemical & Substance for Abstract: Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells. Information

Substance Name: Glucose

Registry Number: 50-99-7

Grant and Affiliation Information for Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells.

AFFILIATION: Department of Neurology, Taipei Medical University, Taipei, Taiwan.

Country: United States

AGENCY: United States NINDS

GRANT: NS40525

ACRONYM: NS

MEDLINETA: J Cereb Blood Flow Metab

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