Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness.

Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. Abstract Text:

Kimon A G Karatzas,Mark A Webber,Frieda Jorgensen,Martin J Woodward,Laura J V Piddock,Tom J Humphrey,Kimon A G Karatzas,Mark A Webber,Frieda Jorgensen,Martin J Woodward,Laura J V Piddock,Tom J Humphrey,Kimon A G Karatzas,Mark A Webber,Frieda Jorgensen,Martin J Woodward,Laura J V Piddock,Tom J Humphrey,

Objectives To study how disinfectants affect antimicrobial susceptibility and phenotype of Salmonella enterica serovar Typhimurium SL1344. Methods Wild-type strain SL1344 and its isogenic gyrA mutant were passaged daily for 7 days in subinhibitory concentrations, and separately for 16 days in gradually increasing concentrations of a quaternary ammonium disinfectant containing formaldehyde and glutaraldehyde (QACFG), an oxidizing compound blend (OXC), a phenolic tar acids-based disinfectant (TOP) and triclosan. The MICs of antimicrobials and antibiotics for populations and representative isolates and the proportion of cells resistant to the MICs for the wild-type were determined. Expression of acrB gene, growth at 37 degrees C and invasiveness of populations in Caco-2 intestinal epithelial cells were assessed. Results QACFG and triclosan showed the highest selectivity for variants with reduced susceptibility to chloramphenicol, tetracycline, ampicillin, acriflavine and triclosan. Populations treated with the above biocides had reduced invasiveness in Caco-2 cells, and altered growth kinetics. Resistance to disinfectants was observed only after exposure to gradually increasing concentrations of triclosan, accompanied with a 2000-fold increase in its MIC. Growth in OXC and TOP did not affect the MICs of antibiotics, but resulted in the appearance of a proportion of cells resistant to the MIC of acriflavine and triclosan for the wild-type. Randomly selected stable variants from all populations, except the one treated with TOP, over-expressed acrB. Conclusions In vitro exposure to QACFG and triclosan selects for Salmonella Typhimurium cells with reduced susceptibility to several antibiotics. This is associated with overexpression of AcrAB efflux pump, but accompanied with reduced invasiveness.

Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. Publishing Authors By Initials

KA Karatzas,MA Webber,F Jorgensen,MJ Woodward,LJ Piddock,TJ Humphrey,KA Karatzas,MA Webber,F Jorgensen,MJ Woodward,LJ Piddock,TJ Humphrey,KA Karatzas,MA Webber,F Jorgensen,MJ Woodward,LJ Piddock,TJ Humphrey,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of antimicrobial chemotherapy

VOLUME: 60

Page Numbers: 947-55

Journal Abbreviation: J. Antimicrob. Chemother.

ISSN: 0305-7453

DAY: 13

MONTH: 09

YEAR: 2007

Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7513617

Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness.

AFFILIATION: Zoonotic Infections Group, Division of Veterinary Pathology, Infection and Immunity, School of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Antimicrob Chemother

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness Related Publications

• A theoretical model for F-actin remodeling in vascular smooth muscle cells subjected to cyclic stretch.
• Age-related changes in the suppression of serum luteinizing hormone by estradiol-17beta in developing steers.
• Altered biomechanical properties of carotid arteries in two mouse models of muscular dystrophy.
• Ansa-tris(allyl) complexes of alkali metals: tripodal analogues of cyclopentadienyl and ansa-metallocene ligands.
• Biochemomechanics of cerebral vasospasm and its resolution: II. Constitutive relations and model simulations.
• Biomechanics of the porcine basilar artery in hypertension.
• Break-induced loss of heterozygosity in fission yeast: dual roles for homologous recombination in promoting translocations and preventing de novo telomere addition.
• Dispersion of the third-order nonlinear optical properties of an organometallic dendrimer(1).
• Effect of student duty hours policy on teaching and satisfaction of 3rd year medical students.
• Effects of diets containing gossypol on ovarian histology, function and fertility in prepubertal beef heifers.
• Electrophoretic studies of serum mucoproteins.
• Fructose and citric acid assay in the secretions of the accessory glands of reproduction as indicator tests of male sex hormone activity.
• Health-related quality of life after transient ischemic attack and minor stroke: Is medical or surgical treatment influential?
• Influence of repeated low doses of gonadotrop in releasing hormone on postpartum interval and serum luteinizing hormone in Brahman cross cows.
• Letter to the editor.
• Pneumonia in north-west London, 1942-4; pneumonias not attributable to specific bacterial infection.
• Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness.
• Recommendations for the reporting of prostate carcinoma.
• Recommendations for the reporting of prostate carcinoma: association of directors of anatomic and surgical pathology.
• Regulation of cell cycle and stress responses to hydrostatic pressure in fission yeast.
• Reversal of sex in females of genotype WW in the axolotl (Siredon or Ambystoma mexicanum) and its bearing upon the role of the Z chromosomes in the development of the testis.
• Safety of an attenuated West Nile virus vaccine, live Flavivirus chimera in horses.
• Stress-strain behavior of the passive basilar artery in normotension and hypertension.
• The adenosinetriphosphatase activity of myosins from marine animals.
• The pathogenesis of glomerulonephritis; a reinvestigation of the auto-immunization hypothesis.
• The persistence and character of stranded oil on coarse-sediment beaches.
• The relation of ESP to mode of drawing.
• The rural health program.
• The utilization of nicotinic acid by pregnant women.
• What do you think of midwife-led units?