Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability?

Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? Research Abstract Details 

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Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? Abstract Text:

Andrew T Pennock,Ferdinand Wagner,Catherine M Robertson,Frederick L Harwood,William D Bugbee,David Amiel,

Osteochondral plugs were harvested from eight fresh human femoral condyles within 96 hours of donor death. The plugs were either stored in a serum-free media containing glucose, salts, and amino acids or 10% fetal bovine serum at 4 degrees C. After 28 days of storage, the osteochondral plugs were analyzed for chondrocyte viability and viable cell density using confocal microscopy, proteoglycan synthesis by (35)SO4 incorporation, and glycosaminoglycan content. Chondrocyte viability and cell density were significantly lower in grafts stored in serum-free media compared to fetal bovine serum, 27% versus 68% (P < .001) and 3250 cells/mm3 versus 8960 cells/mm3, respectively (P < .001). The metabolic activity determined by proteoglycan synthesis was significantly better in the specimens stored in fetal bovine serum (P < .01). No significant difference was detected between the glycosaminoglycan content in any of the specimens. These data suggest that the quality of osteochondral allografts as measured by chondrocyte viability, viable cell density, and proteoglycan synthesis is superior after storage in fetal bovine serum versus serum-free media. These results must be taken cautiously, however, as the clinical ramifications of storage in fetal bovine serum, including potential infectious disease transmission risks and immunogenic factors, have yet to be studied.

Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? Publishing Authors By Initials

AT Pennock,F Wagner,CM Robertson,FL Harwood,WD Bugbee,D Amiel,

For similar equipment and supplies: transplants research abstracts see: equipment and supplies: transplants research

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Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The journal of knee surgery

VOLUME: 19

Page Numbers: 265-72

Journal Abbreviation:

ISSN: 1538-8506

DAY: 3

MONTH: Oct

YEAR: 2006

Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101137599

Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? Keywords Mesh Terms:

KEYWORDS: Transplants

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? Information

Substance Name: Serum Albumin, Bovine

Registry Number: 0

Grant and Affiliation Information for Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability?

AFFILIATION: Department of Orthopedic Surgery, University of California, San Diego 92093-0630, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: AR49380

ACRONYM: AR

MEDLINETA: J Knee Surg

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