Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa.

Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa. Research Abstract Details 

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Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa. Abstract Text:

Katsushi Nishimaki,Kaori Okuyama,Shinji Okada,Toshio Hattori,Motoaki Takayanagi,Isao Ohno,Katsushi Nishimaki,Kaori Okuyama,Shinji Okada,Toshio Hattori,Motoaki Takayanagi,Isao Ohno,

BACKGROUND AND OBJECTIVES: The pathogenesis of airway inflammation in diffuse panbronchiolitis (DPB) is unknown. Neutrophil survival-enhancing activity, partially mediated by granulocyte-macrophage colony-stimulating factor (GM-CSF), has been shown in the sputum from DPB patients. This study investigated the mechanisms of GM-CSF expression in the airways of DPB patients. This involved examining the effects of mucoid Pseudomonas aeruginosa strains derived from chronically colonized patients with DPB on neutrophil survival and GM-CSF expression. METHODS: Neutrophils from healthy subjects were cultured with the culture supernatants of P. aeruginosa isolated from sputum of DPB patients in the presence or absence of anti-GM-CSF and anti-GM-CSF receptor (alpha chain) antibodies, and viable neutrophils were counted daily. GM-CSF gene expression in neutrophils was evaluated by RT-PCR. RESULTS: Neutrophils cultured with the culture supernatants showed significantly prolonged survival, compared with neutrophils cultured with the control broth. The neutrophil survival-enhancing activity in the culture supernatants was lost by heating. The enhanced survival of neutrophils was abolished in the presence of anti-GM-CSF and anti-GM-CSF receptor (alpha chain) antibodies. GM-CSF mRNA was detected in neutrophils cultured with the bacterial supernatants, but not in those with the control broth. CONCLUSION: P. aeruginosa-derived factors (rich in proteins) stimulated neutrophils to synthesize GM-CSF, which enhanced neutrophil survival in an autocrine/paracrine fashion.

Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa. Publishing Authors By Initials

K Nishimaki,K Okuyama,S Okada,T Hattori,M Takayanagi,I Ohno,K Nishimaki,K Okuyama,S Okada,T Hattori,M Takayanagi,I Ohno,

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Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Respirology (Carlton, Vic.)

VOLUME: 12

Page Numbers: 664-9

Journal Abbreviation: Respirology

ISSN: 1323-7799

DAY: 18

MONTH: Sep

YEAR: 2007

Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa. Information

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LANGUAGE: eng

NlmUniqueID: 9616368

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Grant and Affiliation Information for Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa.

AFFILIATION: Department of Respiratory and Infectious Diseases, Tohoku University Graduate School of Medicine, Sendai, Japan.

Country: Australia

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MEDLINETA: Respirology

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