Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress.

Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Abstract Text:

Navasona Krishnan,Martin B Dickman,Donald F Becker,

The potential of proline to suppress reactive oxygen species (ROS) and apoptosis in mammalian cells was tested by manipulating intracellular proline levels exogenously and endogenously by overexpression of proline metabolic enzymes. Proline was observed to protect cells against H(2)O(2), tert-butyl hydroperoxide, and a carcinogenic oxidative stress inducer but was not effective against superoxide generators such as menadione. Oxidative stress protection by proline requires the secondary amine of the pyrrolidine ring and involves preservation of the glutathione redox environment. Overexpression of proline dehydrogenase (PRODH), a mitochondrial flavoenzyme that oxidizes proline, resulted in 6-fold lower intracellular proline content and decreased cell survival relative to control cells. Cells overexpressing PRODH were rescued by pipecolate, an analog that mimics the antioxidant properties of proline, and by tetrahydro-2-furoic acid, a specific inhibitor of PRODH. In contrast, overexpression of the proline biosynthetic enzymes Delta(1)-pyrroline-5-carboxylate (P5C) synthetase (P5CS) and P5C reductase (P5CR) resulted in 2-fold higher proline content, significantly lower ROS levels, and increased cell survival relative to control cells. In different mammalian cell lines exposed to physiological H(2)O(2) levels, increased endogenous P5CS and P5CR expression was observed, indicating that upregulation of proline biosynthesis is an oxidative stress response.

Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Publishing Authors By Initials

N Krishnan,MB Dickman,DF Becker,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Free radical biology & medicine

VOLUME: 44

Page Numbers: 671-81

Journal Abbreviation: Free Radic. Biol. Med.

ISSN: 0891-5849

DAY: 12

MONTH: 11

YEAR: 2007

Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8709159

Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress.

AFFILIATION: Department of Biochemistry, University of Nebraska-Lincoln, N258 Beadle Center, Lincoln, NE 68588, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Free Radic Biol Med

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress Related Publications

• A combined intradural presigmoid-transtransversarium-transcondylar approach to the whole clivus and anterior craniospinal region: anatomic study.
• A trivial sign to rare genetic disorder.
• Attending to a misoriented word causes the eyeball to rotate in the head.
• BOLD-MRI assessment of intrarenal oxygenation and oxidative stress in patients with chronic kidney allograft dysfunction.
• Cerebral ventricular changes associated with transitions between normal cognitive function, mild cognitive impairment, and dementia.
• Dial-a-doc gets boost. JCAHO issues first official telemedicine endorsement.
• Effects of Plant Bioregulators on Valepotriate Production of Cell Suspension Cultures of Valeriana wallichii and Fedia cornucopiae.
• Evidence-bases medicine: What is the evidence?
• Expression of the zebrafish recognition molecule F3/F11/contactin in a subset of differentiating neurons is regulated by cofactors associated with LIM domains.
• Glucose levels in the normal range predict incident diabetes in families with premature coronary heart disease.
• HPCL Separation and Quantitative Determination of Valepotriates from Valeriana kilimandascharica.
• Inhibition of TNF-alpha reduces myocardial injury and proinflammatory pathways following ischemia-reperfusion in the dog.
• Intensive caring. He's a physician-lawyer-consultant, and his patients are typically small hospitals on life support.
• Methylated flavonoid-aglycones from "matricariae flos".
• Middle cranial fossa transtemporal approach to the intrapetrous internal carotid artery.
• Migration, cultural bereavement and cultural identity.
• PlexinA3 restricts spinal exit points and branching of trunk motor nerves in embryonic zebrafish.
• Redox-induced changes in flavin structure and roles of flavin N(5) and the ribityl 2'-OH group in regulating PutA--membrane binding.
• Reinvestigation of the phenylpropanoids from the roots of pimpinella species.
• The CARI guidelines. Kidney stones: cystine stones.
• The CARI guidelines. Kidney stones: uric acid stones.
• The first extracellular loop of the Saccharomyces cerevisiae G protein-coupled receptor Ste2p undergoes a conformational change upon ligand binding.
• The prevalence of microalbuminuria and ECG left ventricular hypertrophy in hypertensive patients in private practices in South Africa.
• The role of friendship in the lives of male and female adolescents: does diabetes make a difference?
• Treatment of sleep dysfunction and psychiatric disorders.
• Unmitigated communion and health among adolescents with and without diabetes: the mediating role of eating disturbances.
• Vascular access outcomes and medication use: a USRDS study.
• Ventricular volume and dementia progression in the Cardiovascular Health Study.
• Voluntary ethanol drinking in mice and ethanol concentrations in the nucleus accumbens.
• [In Process Citation]