Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea.

Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea. Research Abstract Details 

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Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea. Abstract Text:

J W Hong,J J Liu,J S Lee,R R Mohan,R R Mohan,D J Woods,Y G He,S E Wilson,

PURPOSE: To determine the effect of interleukin (IL)-1alpha and tumor necrosis factor (TNF)-alpha on cytokine, chemokine, and receptor expression in corneal stromal cells; the effect of corneal scrape injury on monocyte chemotactic and activating factor (MCAF) expression and monocyte-macrophage influx into the stroma; and the effect of MCAF and granulocyte colony-stimulating factor (G-CSF) microinjection on inflammatory cell infiltration into the stroma. METHODS: Gene array technology was used to evaluate changes in cytokine, chemokine, and receptor gene expression in stromal fibroblasts in response to IL-1alpha and TNFalpha. Expression of MCAF mRNA and protein was monitored with an RNase protection assay and Western blot analysis, respectively. Keratocyte MCAF protein expression in the rabbit cornea was detected with immunocytochemistry. After epithelial scrape injury, monocytes-macrophages were detected in rabbit corneas, by immunocytochemistry for monocyte-macrophage antigen. Inflammatory cell infiltration after MCAF and G-CSF microinjection into the stroma of mouse corneas was monitored with hematoxylin and eosin staining. RESULTS: IL-1alpha or TNFalpha upregulated the expression of several proinflammatory chemokines in stromal fibroblasts in culture. These included G-CSF, MCAF, neutrophil-activating peptide (ENA-78), and monocyte-derived neutrophil chemotactic factor (MDNCF). MCAF mRNA upregulation was confirmed by RNase protection assay, and MCAF protein was detected by Western blot analysis. MCAF protein was detected in keratocytes at 4 hours and 24 hours after epithelial injury, but not in keratocytes in the unwounded cornea. Corneal epithelial injury triggered the influx of monocytes-macrophages into the corneal stroma in the rabbit. Microinjection of MCAF and G-CSF into mouse cornea resulted in the influx of monocytes-macrophages and granulocytes, respectively, into the stroma. CONCLUSIONS: Proinflammatory chemokine induction in keratocytes is mediated by IL-1alpha and TNFalpha. The proinflammatory chemokines produced by the keratocytes probably trigger the influx of inflammatory cells into the stroma after epithelial injury associated with corneal surgery, contact lenses, or trauma.

Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea. Publishing Authors By Initials

JW Hong,JJ Liu,JS Lee,RR Mohan,RR Mohan,DJ Woods,YG He,SE Wilson,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

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Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Investigative ophthalmology & visual science

VOLUME: 42

Page Numbers: 2795-803

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 19

MONTH: Nov

YEAR: 2001

Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7703701

Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea. Information

Substance Name: Granulocyte Colony-Stimulating Factor

Registry Number: 143011-72-7

Grant and Affiliation Information for Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea.

AFFILIATION: Department of Ophthalmology, University of Washington School of Medicine, Seattle 98195-6485, USA.

Country: United States

AGENCY: United States NEI

GRANT: EYO1730

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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