Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method.

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Research Abstract Details 

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Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Abstract Text:

Xiangdong Meng,Stacey Thibodeau-Beganny,Tao Jiang,J Keith Joung,Scot A Wolfe,

The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify individual fingers that possess a particular DNA-binding specificity from a randomized library. In these experiments, each finger is selected in the context of a constant finger framework that ensures the identification of clones with a desired specificity by properly positioning the randomized finger on the DNA template. Following a successful selection, multiple zinc-finger clones are typically recovered that share similarities in the sequences of their DNA-recognition helices. In principle, each of the clones isolated from a selection is a candidate for assembly into a larger multi-finger protein, but to date a high-throughput method for identifying the most specific candidates for incorporation into a final multi-finger protein has not been available. Here we describe the development of a specificity profiling system that facilitates rapid and inexpensive characterization of engineered zinc-finger modules. Moreover, we demonstrate that specificity data collected using this system can be employed to rationally design zinc fingers with improved DNA-binding specificities.

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Publishing Authors By Initials

X Meng,S Thibodeau-Beganny,T Jiang,JK Joung,SA Wolfe,

For similar zinc fingers research abstracts see: zinc fingers research

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Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: e81

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 30

MONTH: 05

YEAR: 2007

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Information

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LANGUAGE: eng

NlmUniqueID: 411011

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Keywords Mesh Terms:

KEYWORDS: Zinc Fingers

MESH TERMS: chemistry

Chemical & Substance for Abstract: Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method.

AFFILIATION: Program in Gene Function and Expression, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA.

Country: England

AGENCY: United States NIGMS

GRANT: R01 GM072621

ACRONYM: GM

MEDLINETA: Nucleic Acids Res

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