Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier.

Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier. Research Abstract Details 

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Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier. Abstract Text:

Mihee Kim,Chul-Ho Yun,Seong-Ki Park,Jung-Hoon Seo,Taeho Ahn,Mihee Kim,Chul-Ho Yun,Seong-Ki Park,Jung-Hoon Seo,Taeho Ahn,

We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 microm diam) were conjugated with the peptide, which is known as an epitope region, using a chemical cross-linker and injected into rabbits with adjuvant. By immunizing the animals intradermally twice at two week intervals, an immunoblot assay with anti-sera showed the efficient generation of the PAb.

Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier. Publishing Authors By Initials

M Kim,CH Yun,SK Park,JH Seo,T Ahn,M Kim,CH Yun,SK Park,JH Seo,T Ahn,

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Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biotechnology letters

VOLUME: 29

Page Numbers: 1735-40

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 13

MONTH: 07

YEAR: 2007

Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier. Information

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LANGUAGE: eng

NlmUniqueID: 8008051

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Grant and Affiliation Information for Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier.

AFFILIATION: Department of Molecular Endocrinology, Chonnam National University, Gwangju, 500-757, Korea.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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