Production of mouse monoclonal antibodies against Helicobacter pylori Catalase and mapping the antigenic epitope by phage display library.

Production of mouse monoclonal antibodies against Helicobacter pylori Catalase and mapping the antigenic epitope by phage display library. Research Abstract Details 

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Production of mouse monoclonal antibodies against Helicobacter pylori Catalase and mapping the antigenic epitope by phage display library. Abstract Text:

Yan Li,Yun-Shan Ning,Yun-Dan Wang,Jun Luo,Wenjing Wang,Wen-Qi Dong,Ming Li,Yan Li,Yun-shan Ning,Yun-dan Wang,Jun Luo,Wenjing Wang,Wen-qi Dong,Ming Li,

The Catalase of Helicobacter pylori (H. pylori) helps bacteria to protect themselves from oxygen toxicity and damage and have been identified an immunodominant antigen. To obtain mouse monoclonal antibodies (mAbs) against Catalase and to map its antigenic epitope is potentially to develop a vaccine for prevention and treatment of H. pylori infection. In our study, MAbs were produced by the hybridoma technique using recombinant Catalase-GST as the immunogen and were immunoscreened against phage-displayed random dodecapeptide library (Ph.D.-12). After three rounds of biopanning, 34 phage clones were randomly selected and their specificity to mAb was verified by sandwich and competitive inhibition ELISA. Fifteen phage clones were sequenced and their amino acids were deduced. One mimotope (SVSLPYANLATH) showed good match with Catalase protein at 394-405aa and the serum of mice induced by the phage clone clearly recognized Catalase protein. Our work suggests that the antigenic epitope could be mapped through screening the phage-displayed peptide libraries with mAb and a mimotope of Catalase would provide an alternative approach for the development of a vaccine for H. pylori.

Production of mouse monoclonal antibodies against Helicobacter pylori Catalase and mapping the antigenic epitope by phage display library. Publishing Authors By Initials

Y Li,YS Ning,YD Wang,J Luo,W Wang,WQ Dong,M Li,Y Li,YS Ning,YD Wang,J Luo,W Wang,WQ Dong,M Li,

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Production of mouse monoclonal antibodies against Helicobacter pylori Catalase and mapping the antigenic epitope by phage display library. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Vaccine

VOLUME: 26

Page Numbers: 1263-9

Journal Abbreviation: Vaccine

ISSN: 0264-410X

DAY: 7

MONTH: 12

YEAR: 2007

Production of mouse monoclonal antibodies against Helicobacter pylori Catalase and mapping the antigenic epitope by phage display library. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Production of mouse monoclonal antibodies against Helicobacter pylori Catalase and mapping the antigenic epitope by phage display library.

AFFILIATION: School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, PR China.

Country: Netherlands

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MEDLINETA: Vaccine

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