Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207.

Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207. Research Abstract Details 

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Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207. Abstract Text:

Cheolho Cheong,Juliana Idoyaga,Yoonkyung Do,Maggi Pack,Sung Ho Park,Haekyung Lee,Young-Sun Kang,Jae-Hoon Choi,Jae Y Kim,Anthony Bonito,Kayo Inaba,Sayuri Yamazaki,Ralph M Steinman,Chae Gyu Park,

Langerin CD207 is a type II transmembrane protein. It is responsible for the formation of Birbeck granules, which are intracellular organelles within Langerhans cells, the dendritic cells of stratified squamous epithelia like the epidermis. Because current anti-CD207 antibodies have limitations, we prepared new monoclonals by immunizing rats with the extracellular region of mouse Langerin followed by a boost with enriched Langerhans cells (LCs). We secured a large panel of mAbs, most of which reacted with the carboxy terminal carbohydrate recognition domain. These mAbs could be used to immunoblot and immunoprecipitate mouse Langerin and to stain the cell surface and intracellular pools of CD207 by FACS analysis. Labeling of Birbeck granules was also achieved by immunoelectron microscopy. Anti-CD207 identified LCs in the epidermis and skin draining lymph nodes of BALB/c and C57BL/6 mice, but BALB/c mice had an additional Langerin(+) population in spleen, thymus and mesenteric lymph node. This additional subset had higher levels of CD8 and CD205 than epidermal LCs, and also had a less mature phenotype, i.e., lower MHC II, CD40 and CD86. Subcutaneous injection of IgG but not IgM forms of these new anti-CD207 mAbs led to rapid and selective labeling of the Langerin(+) cells in skin draining lymph nodes as well as spleen. The new IgG anti-CD207 mAbs should be useful for further research on LCs and dendritic cells including an evaluation of the consequences of antigen delivery within anti-CD207 mAbs in vivo.

Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207. Publishing Authors By Initials

C Cheong,J Idoyaga,Y Do,M Pack,SH Park,H Lee,YS Kang,JH Choi,JY Kim,A Bonito,K Inaba,S Yamazaki,RM Steinman,CG Park,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred wf research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains: rats, inbred wf research

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MEDLINE DATE:

Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Journal of immunological methods

VOLUME: 324

Page Numbers: 48-62

Journal Abbreviation: J. Immunol. Methods

ISSN: 0022-1759

DAY: 24

MONTH: 05

YEAR: 2007

Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1305440

Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred WF

MESH TERMS: immunology

Chemical & Substance for Abstract: Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207. Information

Substance Name: Mannose-Binding Lectins

Registry Number: 0

Grant and Affiliation Information for Production of monoclonal antibodies that recognize the extracellular domain of mouse langerin/CD207.

AFFILIATION: Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.

Country: Netherlands

AGENCY: United States NIAID

GRANT: AI 40045

ACRONYM: AI

MEDLINETA: J Immunol Methods

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