Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium.

Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium. Research Abstract Details 

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Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium. Abstract Text:

Dengchao Li,Shiwei Cheng,Dongzhi Wei,Yuhong Ren,Derong Zhang,Dengchao Li,Shiwei Cheng,Dongzhi Wei,Yuhong Ren,Derong Zhang,Dengchao Li,Shiwei Cheng,Dongzhi Wei,Yuhong Ren,Derong Zhang,

A new approach has been developed for the production of enantiomerically pure (S)-beta-phenylalanine (S-BPA) and (R)-beta-phenylalanine in aqueous medium based on enantioselective acylation and hydrolysis properties of penicillin G acylase from Escherichia coli. The acylation reaction was highly preferential for the acylation of (R)-BPA to form N-phenylacetyl-(R)-BPA using phenylacetamide as an acyl donor, which was separated and then hydrolyzed to (R)-BPA by the same enzyme at pH 7.5. The optimal acylation reaction was at pH 10, 25 degrees C with a 2:1 molar ratio of phenylacetamide to BPA, 8 IU ml(-1) enzyme and 150 mM BPA. These resulted in a conversion of about 50% BPA; enantiomeric excess of (S)-BPA and (R)-BPA separated were 98 and 99%, respectively.

Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium. Publishing Authors By Initials

D Li,S Cheng,D Wei,Y Ren,D Zhang,D Li,S Cheng,D Wei,Y Ren,D Zhang,D Li,S Cheng,D Wei,Y Ren,D Zhang,

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Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology letters

VOLUME: 29

Page Numbers: 1825-30

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 27

MONTH: 07

YEAR: 2007

Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium.

AFFILIATION: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, P.R. China, dzhwei@ecust.edu.cn.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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