Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 599. The rapidly growing microalga had much higher levels (58%) of deuterium substitution relative to previously reported (9-15%) natural sources of lutein.
Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods. Publishing Authors By Initials
Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods. Journal Published:
PUBLICATION TYPE: Research Support, Non-U.S. Gov
Journal: Biotechnology letters
VOLUME: 28
Page Numbers: 1371-5
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ISSN: 0141-5492
DAY: 6
MONTH: 07
YEAR: 2006
Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods. Information
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LANGUAGE: eng
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Grant and Affiliation Information for Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods.
AFFILIATION: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine, 50 North Medical Drive, Moran Eye Center, Salt Lake City, UT 84132, USA.
Country: Netherlands
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MEDLINETA: Biotechnol Lett
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