Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin.

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. Abstract Text:

K Hinrichs,Y H Choi,D D Varner,D L Hartman,

We evaluated the effect of different activation treatments on the production of blastocysts and foals by nuclear transfer. Donor cells were prepared using roscovitine treatment, which has previously been associated with increased production of viable offspring. All activation treatments were followed by culture in 6-dimethylaminopurine (6-DMAP) for 4 h. In experiment 1, blastocyst production after activation by injection of sperm extract followed by treatment with ionomycin was significantly higher than that for activation with a serial treatment of ionomycin, 6-DMAP, and ionomycin (12.5 vs 2.8%; P < 0.05) and tended to be higher than that for injection of sperm extract alone (3.4%; P = 0.07). In experiment 2, there were no significant differences in blastocyst development among treatments with ionomycin once or twice, sperm extract then ionomycin, or ionomycin then sperm extract (range 4.6-7.3%). Overall, transfer of 26 blastocysts resulted in 16 pregnancies (62%) and 9 live foals (35% of transferred embryos). Treatment with sperm extract followed by ionomycin produced a live foal rate per embryo transferred of 5/10 (50%). One foal died of pneumonia 48 h post partum and one foal died at 1 week of age after complications during induction of anesthesia; the remaining seven foals are currently 10-14 months of age.

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. Publishing Authors By Initials

K Hinrichs,YH Choi,DD Varner,DL Hartman,

For similar therapeutics: reproductive techniques: reproductive techniques, assisted: fertilization in vitro: sperm injections, intracytoplasmic research abstracts see: therapeutics: reproductive techniques: reproductive techniques, assisted: fertilization in vitro: sperm injections, intracytoplasmic research

PUBMED ID PMID:

MEDLINE DATE:

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Reproduction (Cambridge, England)

VOLUME: 134

Page Numbers: 319-25

Journal Abbreviation: Reproduction

ISSN: 1470-1626

DAY: 27

MONTH: Aug

YEAR: 2007

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100966036

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. Keywords Mesh Terms:

KEYWORDS: Sperm Injections, Intracytoplasmic

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. Information

Substance Name: N(6),N(6)-dimethyladenine

Registry Number: 938-55-6

Grant and Affiliation Information for Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin.

AFFILIATION: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA. khinrichs@cvm.tamu.edu

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Reproduction

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin Related Publications

• A simplified gas liquid chromatographic determination for vitamin E in vegetable oils.
• Action of some alkylhydroxybenzenes on pig ascaris in vitro.
• An IgM- and IgG-specific enzyme-linked immunosorbent assay (ELISA) to detect anti-leptospiral immunoglobulins in dogs.
• Artifacts and pitfall errors associated with ultrasound-guided regional anesthesia. Part I: understanding the basic principles of ultrasound physics and machine operations.
• Artifacts and pitfall errors associated with ultrasound-guided regional anesthesia. Part II: a pictorial approach to understanding and avoidance.
• Brief Report: Adults with Mild Autism Spectrum Disorders (ASD): Scores on the Autism Spectrum Quotient (AQ) and Comorbid Psychopathology.
• Buffering of deoxyribonucleotide pool homeostasis by threonine metabolism.
• Early reintervention of compromised free flaps improves success rate.
• Epidemiological aspects of canine leptospirosis in the Netherlands.
• Esterification rates of some saturated and unsaturated fatty acids with glycerol.
• IGF-I measurements in the diagnosis of adult growth hormone deficiency.
• ISMS symposium on medical implications of the current abortion law in Illinois.
• Kinesin spindle protein (KSP) inhibitors. Part 6: Design and synthesis of 3,5-diaryl-4,5-dihydropyrazole amides as potent inhibitors of the mitotic kinesin KSP.
• Kinesin spindle protein (KSP) inhibitors. Part 7: Design and synthesis of 3,3-disubstituted dihydropyrazolobenzoxazines as potent inhibitors of the mitotic kinesin KSP.
• Kinesin spindle protein (KSP) inhibitors. Part 8: Design and synthesis of 1,4-diaryl-4,5-dihydropyrazoles as potent inhibitors of the mitotic kinesin KSP.
• Maternal death study no. 8.
• Maternal death study no. 9.
• Maternal death study. Case report no. 6.
• Maternal death study. Case report no. 7.
• Motor skill performance of school-age children with visual impairments.
• Possible uses of a delayed-action antihistaminic; clinical trials.
• Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin.
• SNPs in candidate genes for hypertension : time for more integrated approaches?
• Scaling up TB DOTS in a fragile state: post-conflict Afghanistan.
• Sterile homopneumothorax caused by softening and perforation of a pulmonary infarct.
• Synthesis and evaluation of substituted benzoisoquinolinones as potent inhibitors of Chk1 kinase.
• TEST REVIEW: PSST! Wanna Buy a Good New Memory Test-Cheap?: The Test of Memory and Learning-2.
• The control of anoxemia during surgical anesthesia with the aid of the oxyhemograph.
• The preparation and measurement of the concentration of dilute bacterial aerosols.
• The role of the electron transport gene SDHC on lifespan and cancer.