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Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes.

Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes. Research Abstract Details 

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  • Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes. Abstract Text:

    p r adalbertoP R Adalberto,a c massabniA C Massabni,a j goulartA J Goulart,j contieroJ Contiero,e c carmonaE C Carmona,l cardelloL Cardello,r montiR Monti,

    Trichoderma reesei FTKO-39 grown at 35 degrees C for 5 d on wheat bran supplemented with MgCl2 and lactose as the carbon source produced two isozymes of beta-galactosidase: BGT I and BGT II. These isozymes were partially purified on a DEAE-Trisacryl column. Both BGT I and BGT II fractions exhibited optimum activity at 65 degrees C, but the pH optima were 4.0 and 6.5, respectively. The isozymes also showed similar thermal stability. However, BGT I was more stable than BGT II in a pH range of 3.0-10.0. At least two different beta-galactosidases are produced by T. reesei, as revealed by the two bands seen on a 6% polyacrylamide gel stained for activity.

    Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes. Publishing Authors By Initials

    pr adalbertoPR Adalberto,ac massabniAC Massabni,aj goulartAJ Goulart,j contieroJ Contiero,ec carmonaEC Carmona,l cardelloL Cardello,r montiR Monti,

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    Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Applied biochemistry and biotechnology

    VOLUME: 133

    Page Numbers: 163-70

    Journal Abbreviation: Appl. Biochem. Biotechnol.

    ISSN: 0273-2289

    DAY: 16

    MONTH: May

    YEAR: 2006

    Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes. Information

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    LANGUAGE: eng

    NlmUniqueID: 8208561

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    Grant and Affiliation Information for Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes.

    AFFILIATION: Department of General Inorganic and Chemistry, Institute of Chemistry, Rua Prof. Francisco Degni, s/n, 14800-900, Araraquara, SP, Brazil.

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: Appl Biochem Biotechnol

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