[Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture]

[Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture] Research Abstract Details 

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[Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture] Abstract Text:

Y Zhang,L Chen,S Wang,C Guo,P Mao,

Ten million splenocytes from the enteric adenovirus (Ad40/41) immunized mice were fused with 3 x 10(6) myeloma cells by electrofusion. Then, the cells were selectively cultured in methyl cellulose semi-solid medium. Eighteen clones of hybridoma were directely picked out from the culture plates and 7 of them could secret high level specific antibodies to enteric adenovirus. So far, the anti-Ad40/41 virus monoclonal antibodies were successfully generated by the protocal of electrofusion accompanied with selective culture in methyl cellulose semi-solid medium.

[Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture] Publishing Authors By Initials

Y Zhang,L Chen,S Wang,C Guo,P Mao,

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[Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua shi yan he lin chuang bing du xue za zhi

VOLUME: 11

Page Numbers: 69-71

Journal Abbreviation: Zhonghua Shi Yan He Lin Chuang

ISSN: 1003-9279

DAY: 28

MONTH: Mar

YEAR: 1997

[Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture] Information

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LANGUAGE: chi

NlmUniqueID: 9602873

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AFFILIATION: Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing 100005.

Country: China

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MEDLINETA: Zhonghua Shi Yan He Lin Chuang

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