Production of a site- and phosphorylation state-specific antibody.

Production of a site- and phosphorylation state-specific antibody. Research Abstract Details 

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Production of a site- and phosphorylation state-specific antibody. Abstract Text:

Hidemasa Goto,Masaki Inagaki,Hidemasa Goto,Masaki Inagaki,Hidemasa Goto,Masaki Inagaki,

Protein phosphorylation plays important roles in various aspects of cellular events. Visualization of site-specific phosphorylation in cells is of great importance not only to analyze spatial and temporal distribution but also to investigate biological function. Now, site- and phosphorylation state-specific antibodies are widely utilized as the most powerful tools for these analyses. This protocol details a method to produce the polyclonal version of such an antibody by immunizing a synthetic phosphopeptide corresponding to a protein phosphorylated at targeted site(s). This protocol is also applicable to the production of other types of antibodies, which specifically recognize the site-specific modification, such as acetylation, methylation and proteolysis. The protocol can be completed in 2-3 months.

Production of a site- and phosphorylation state-specific antibody. Publishing Authors By Initials

H Goto,M Inagaki,H Goto,M Inagaki,H Goto,M Inagaki,

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Production of a site- and phosphorylation state-specific antibody. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature protocols

VOLUME: 2

Page Numbers: 2574-81

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ISSN: 1750-2799

DAY: 19

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Production of a site- and phosphorylation state-specific antibody.

AFFILIATION: Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464-8681, Japan.

Country: England

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MEDLINETA: Nat Protoc

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