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[Production and some properties of a beta-glucanase from Trichoderma sp. GXC]

[Production and some properties of a beta-glucanase from Trichoderma sp. GXC] Research Abstract Details 

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  • [Production and some properties of a beta-glucanase from Trichoderma sp. GXC] Abstract Text:

    j sunJ Sun,w liW Li,s guS Gu,

    The factors affecting Trichoderma sp. GXC for beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase, EC 3.2.1.73) production have been investigated, the optimal conditions were that carbon and nitrogen sources were bran and ammonium sulfate, respectively, initial pH 4.0-5.0, shaken at 30 degrees C for 44 h. beta-glucanase was purified to electrophoretic homogeneity by using ammonium sulfate precipitation, Sephadex G-100 and DEAE-Sephadex A-50 chromatography. It's molecular weight was 35 kD by SDS-PAGE, and the enzyme functioned optimally at pH6.0 and 50 degrees C, respectively. In addition, the enzyme was stable at pH 4.0-5.0 and below 40 degrees C. The beta-glucanase activity was significantly inhibited by Fe3+ and stimulated by Co2+, respectively.

    [Production and some properties of a beta-glucanase from Trichoderma sp. GXC] Publishing Authors By Initials

    j sunJ Sun,w liW Li,s guS Gu,

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    PUBMED ID PMID:

    MEDLINE DATE:

    [Production and some properties of a beta-glucanase from Trichoderma sp. GXC] Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Wei sheng wu xue bao = Acta microbiologica Sinica

    VOLUME: 41

    Page Numbers: 457-62

    Journal Abbreviation: Wei Sheng Wu Xue Bao

    ISSN: 0001-6209

    DAY: 29

    MONTH: Aug

    YEAR: 2001

    [Production and some properties of a beta-glucanase from Trichoderma sp. GXC] Information

    Number of References:

    LANGUAGE: chi

    NlmUniqueID: 21610860

    [Production and some properties of a beta-glucanase from Trichoderma sp. GXC] Keywords Mesh Terms:

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    Grant and Affiliation Information for [Production and some properties of a beta-glucanase from Trichoderma sp. GXC]

    AFFILIATION: Institute of Feed Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China.

    Country: China

    China Research PublicationChina Research Publication

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    MEDLINETA: Wei Sheng Wu Xue Bao

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