Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine.

Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. Research Abstract Details 

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Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. Abstract Text:

Zheng-You Yang,Won-Bo Shim,Min-Gon Kim,Kyu-Ho Lee,Keun-Sung Kim,Kwang-Yup Kim,Cheol-Ho Kim,Sang-Do Ha,Duck-Hwa Chung,Zheng-You Yang,Won-Bo Shim,Min-Gon Kim,Kyu-Ho Lee,Keun-Sung Kim,Kwang-Yup Kim,Cheol-Ho Kim,Sang-Do Ha,Duck-Hwa Chung,

A monoclonal antibody (mab) against the antimicrobial sulfamethazine was prepared and characterized by an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA). Sulfamethazine in the range of 0.2 and 45 ng/ml could be determined with the mab by IC-ELISA. cDNAs encoding a variable heavy chain and variable light chain of the mab were cloned to produce recombinant antibodies using phage display technology. Following phage rescue and three rounds of panning, a single-chain variable fragment (scFv) antibody with high sulfamethazine-binding affinity was obtained. ELISA analysis revealed that scFv antibody and parent mab showed similar, but not identical, characteristics. The IC50 value by IC-ELISA with scFv antibody was 4.8 ng/ml, compared with 1.6 ng/ml with the parent mab. Performances of the assays in the presence of milk matrix were compared; the mab-based assay was less affected than the scFv-based assay. Sixty milk samples were analyzed by mab-based IC-ELISA, and four samples were sulfamethazine positive; these results were favorably correlated with those obtained by HPLC.

Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. Publishing Authors By Initials

ZY Yang,WB Shim,MG Kim,KH Lee,KS Kim,KY Kim,CH Kim,SD Ha,DH Chung,ZY Yang,WB Shim,MG Kim,KH Lee,KS Kim,KY Kim,CH Kim,SD Ha,DH Chung,

For similar organic chemicals: amides: sulfonamides: sulfanilamides: sulfamethazine research abstracts see: organic chemicals: amides: sulfonamides: sulfanilamides: sulfamethazine research

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Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 571-8

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 3

MONTH: Apr

YEAR: 2007

Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9431852

Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. Keywords Mesh Terms:

KEYWORDS: Sulfamethazine

MESH TERMS: immunology

Chemical & Substance for Abstract: Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. Information

Substance Name: Sulfamethazine

Registry Number: 57-68-1

Grant and Affiliation Information for Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine.

AFFILIATION: Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju 660-701, Korea.

Country: Korea (South)

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MEDLINETA: J Microbiol Biotechnol

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