[Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes]

[Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes] Research Abstract Details 

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[Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes] Abstract Text:

V P Chekhonin,Iu A Zhirkov,O I Gurina,S V Lebedev,T B Dmitrieva,A V Semenova,T V Iaglova,A E Riabinina,

PEGylated (stealth) immunoliposomes covalently linked to antibodies against human gliofibrillary acidic protein (GFAP) were prepared by coupling the thiolated monoclonal anti-GFAP antibodies, D4, with a maleimide derivative of the phosphatidyl ethanolamine of the liposomal membrane. Depending on the initial protein-to-lipid ratios, the immunoliposomes prepared (with a diameter of about 70 nm) were coupled with 60 to 240 molecules of the antibodies. In vitro experiments with cultures of the embryonic rat brain astrocytes demonstrated a specific binding of these immunoliposomes, which could be inhibited by the preincubation of the cells with free non-coupled D4 but not with non-specific antibodies. Administered intravenously into rats, the immunoliposomes exhibited a kinetic behaviour typical for the PEGylated liposomes: after 24 h, approximately 20% of the doses injected were still present in the blood; the elimination rate constants were 0.05-0.09 h(-1), the elimination half-lives were 8-15 h. With such a systemic longevity, as well as with such a specificity, these immunoliposomes, non-penetrating through intact blood-brain barrier (BBB), should be useful in delivering pharmacological agents to glial brain tumours (which continue to express GFAP) or to other pathological loci in the brain with a partially disintegrated BBB.

[Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes] Publishing Authors By Initials

VP Chekhonin,IuA Zhirkov,OI Gurina,SV Lebedev,TB Dmitrieva,AV Semenova,TV Iaglova,AE Riabinina,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, wistar research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, wistar research

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[Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomedit?sinskai?a khimii?a

VOLUME: 51

Page Numbers: 276-86

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DAY: 3

MONTH: 04

YEAR: 2008

[Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes] Information

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LANGUAGE: rus

NlmUniqueID: 101196966

[Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes] Keywords Mesh Terms:

KEYWORDS: Rats, Wistar

MESH TERMS: chemistry

Chemical & Substance for Abstract: [Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes] Information

Substance Name: Polyethylene Glycols

Registry Number: 0

Grant and Affiliation Information for [Production and characteristics of PEGylated immunoliposome transport vectors specific for neural tissue astrocytes]

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Country: Russia (Federation)

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MEDLINETA: Biomed Khim

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