Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer.

Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer. Research Abstract Details 

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Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer. Abstract Text:

M S Simms,A Murray,G Denton,D P Scholfield,M R Price,A C Perkins,M C Bishop,

Current radiological techniques for staging bladder cancer are inaccurate, especially in the identification of pelvic lymph node metastases. Immunoscintigraphy has the potential to offer improved staging for bladder cancer. The aim of this study was to label the anti-MUC1 monoclonal antibody C595 with 99mtechnetium (Tc), the most widely used diagnostic radionuclide, and assess the potential of the resultant conjugate for intravenous immunoscintigraphy of bladder cancer. A direct, reduction-mediated technique was used to label the antibody. The resultant conjugate was shown to be highly immunoreactive, stable and bound specifically to MUC1. The ability of the conjugate to bind to bladder tumours was demonstrated in an ex vivo model where the mean tumour:normal urothelial uptake was 5.7:1 and by intravesical administration in patients with bladder cancer where the mean tumour:normal urothelial uptake was 20.4:1. The ability of the conjugate to localise MUC1-expressing tumours was demonstrated in a nude mouse xenograft model. A conjugate of 99mTc-C595 has been produced and characterised, and it may be suitable for intravenous immunoscintigraphy, a potential novel staging tool for bladder cancer.

Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer. Publishing Authors By Initials

MS Simms,A Murray,G Denton,DP Scholfield,MR Price,AC Perkins,MC Bishop,

For similar neoplasms: neoplasms by site: urogenital neoplasms: urologic neoplasms: urinary bladder neoplasms research abstracts see: neoplasms: neoplasms by site: urogenital neoplasms: urologic neoplasms: urinary bladder neoplasms research

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Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Urological research

VOLUME: 29

Page Numbers: 13-9

Journal Abbreviation: Urol. Res.

ISSN: 0300-5623

DAY: 1

MONTH: Feb

YEAR: 2001

Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer. Information

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LANGUAGE: eng

NlmUniqueID: 364311

Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer. Keywords Mesh Terms:

KEYWORDS: Urinary Bladder Neoplasms

MESH TERMS: radionuclide imaging

Chemical & Substance for Abstract: Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer. Information

Substance Name: Technetium

Registry Number: 7440-26-8

Grant and Affiliation Information for Production and characterisation of a C595 antibody-99mTc conjugate for immunoscintigraphy of bladder cancer.

AFFILIATION: Department of Urology, Nottingham City Hospital, Hucknall Road, Nottingham NG5 1PB, UK. msimms@gertrude42.freeserve.co.uk

Country: Germany

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MEDLINETA: Urol Res

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