Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1.

Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1. Research Abstract Details 

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Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1. Abstract Text:

The role of human FEN1 (flap endonuclease-1), an RTH1 (RAD two homolog-1) class nuclease, in the replication of human immunodeficiency virus (HIV) type 1 has been examined using model substrates. FEN1 is able to endonucleolytically cleave a primer annealed to a template, but with a 5'-unannealed tail. The HIV (+)-strand is synthesized as two discontinuous segments, with the upstream segment displacing the downstream segment to form a central (+)-strand overlap. Given a substrate with the exact HIV nucleotide sequence, FEN1 was able to remove the overlap. After extension of the upstream primer with DNA polymerase epsilon, human DNA ligase I was able to complete the continuous double strand as would occur for an integrated provirus. FEN1 may represent a target for new therapeutic interventions.

Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1. Publishing Authors By Initials

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 273

Page Numbers: 28740-5

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 30

MONTH: Oct

YEAR: 1998

Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: chemistry

Chemical & Substance for Abstract: Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1. Information

Substance Name: DNA ligase (ATP)

Registry Number: EC 6.5.1.1

Grant and Affiliation Information for Processing of an HIV replication intermediate by the human DNA replication enzyme FEN1.

AFFILIATION: Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.

Country: UNITED STATES

AGENCY: United States NIGMS

GRANT: GM49573

ACRONYM: GM

MEDLINETA: J Biol Chem

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