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Processing and cryopreservation for 1963 units of human umbilical cord blood.

Processing and cryopreservation for 1963 units of human umbilical cord blood. Research Abstract Details 

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  • Processing and cryopreservation for 1963 units of human umbilical cord blood. Abstract Text:

    jin-hui liuJin-Hui Liu,ji heJi He,fa-ming zhuFa-Ming Zhu,li-xing yanLi-Xing Yan,

    The study was aimed to establish a standard procedure for human umbilical cord blood bank. The hematopoietic nucleated cells in cord blood were processed by using sedimentation and centrifugation method. After finishing CD34(+) cell counting, hematopoietic progenitor cell assay, microbial culture, infectious disease test and HLA typing, cord blood units were stored in the liquid nitrogen for further application. The results showed that nucleated cells of cord blood were (10.94 +/- 2.74) x 10(8) per unit; recovery rate of nucleated cells was (79.82 +/- 17.76)%. CD34(+) cells in cord blood were counted as (51.62 +/- 30.53) x 10(5) per unit. Eight units of cord blood were thawed after two years of cryopreservation, the recovery rate of nucleated cells, CD34(+) cells and CFU-GM were (91.4 +/- 6.0)%, (84.6 +/- 20.0)% and (85.8 +/- 14.9)% respectively. It is suggested that the methods and procedure reported for processing and cryopreservation of hematopoietic stem/progenitor cells in the human umbilical cord blood is effective.

    Processing and cryopreservation for 1963 units of human umbilical cord blood. Publishing Authors By Initials

    jh liuJH Liu,j heJ He,fm zhuFM Zhu,lx yanLX Yan,

    For similar abstracts research abstracts see: abstracts research

    PUBMED ID PMID:

    MEDLINE DATE:

    Processing and cryopreservation for 1963 units of human umbilical cord blood. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing

    VOLUME: 13

    Page Numbers: 143-6

    Journal Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za

    ISSN: 1009-2137

    DAY: 7

    MONTH: Feb

    YEAR: 2005

    Processing and cryopreservation for 1963 units of human umbilical cord blood. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 101084424

    Processing and cryopreservation for 1963 units of human umbilical cord blood. Keywords Mesh Terms:

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    Grant and Affiliation Information for Processing and cryopreservation for 1963 units of human umbilical cord blood.

    AFFILIATION: Blood Center of Zhejiang Province, Hangzhou 310006, China.

    Country: China

    China Research PublicationChina Research Publication

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    MEDLINETA: Zhongguo Shi Yan Xue Ye Xue Za

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