Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy.

Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Research Abstract Details 

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Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Abstract Text:

Katsunori Nakagawa,Satoru Suzuki,Ritsuko Fujii,Alastair T Gardiner,Richard J Cogdell,Mamoru Nango,Hideki Hashimoto,

Stark spectroscopy is a powerful technique to investigate the electrostatic interactions between pigments as well as between the pigments and the proteins in photosynthetic pigment-protein complexes. In this study, Stark spectroscopy has been used to determine two nonlinear optical parameters (polarizability change Tr(Deltaalpha) and static dipole-moment change |Deltamu| upon photoexcitation) of isolated and of reconstituted LH1 complexes from the purple photosynthetic bacterium, Rhodospirillum (Rs.) rubrum. The integral LH1 complex was prepared from Rs. rubrum S1, while the reconstituted complex was assembled by addition of purified carotenoid (all-trans-spirilloxanthin) to the monomeric subunit of LH1 from Rs. rubrum S1. The reconstituted LH1 complex has its Q(y) absorption maximum at 878 nm. This is shifted to the blue by 3 nm in comparison to the isolated LH1 complex. The energy transfer efficiency from carotenoid to bacteriochlorophyll a (BChl a), which was determined by fluorescence excitation spectroscopy of the reconstituted LH1 complex, is increased to 40%, while the efficiency in the isolated LH1 complex is only 28%. Based on the differences in the values of Tr(Deltaalpha) and |Deltamu|, between these two preparations, we can calculate the change in the electric field around the BChl a molecules in the two situations to be E (Delta) approximately 3.4 x 10(5) [V/cm]. This change can explain the 3 nm wavelength shift of the Q(y) absorption band in the reconstituted LH1 complex.

Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Publishing Authors By Initials

K Nakagawa,S Suzuki,R Fujii,AT Gardiner,RJ Cogdell,M Nango,H Hashimoto,

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Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Photosynthesis research

VOLUME: 95

Page Numbers: 339-44

Journal Abbreviation: Photosyn. Res.

ISSN: 0166-8595

DAY: 3

MONTH: 10

YEAR: 2007

Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy.

AFFILIATION: Department of Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Nagoya, 466-8555, Japan.

Country: Netherlands

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MEDLINETA: Photosynth Res

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