Prion infection of muscle cells in vitro.

Prion infection of muscle cells in vitro. Research Abstract Details 

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Prion infection of muscle cells in vitro. Abstract Text:

Wendy M Dlakic,Eric Grigg,Richard A Bessen,

The prion agent has been detected in skeletal muscle of humans and animals with prion diseases. Here we report scrapie infection of murine C2C12 myoblasts and myotubes in vitro following coculture with a scrapie-infected murine neuroblastoma (N2A) cell line but not following incubation with a scrapie-infected nonneuronal cell line or a scrapie brain homogenate. Terminal differentiation of scrapie-infected C2C12 myoblasts into myotubes resulted in an increase in the expression of the disease-specific prion protein, PrP(Sc). The amount of scrapie infectivity or PrP(Sc) in C2C12 myotubes was comparable to the levels found in scrapie-infected N2A cells, indicating that a high level of infection was established in muscle cells. Subclones of scrapie-infected C2C12 cells produced high levels of PrP(Sc) in myotubes, and the C-terminal C2 polypeptide fragment of PrP(Sc) was found based on deglycosylation and PrP(Sc)-specific immunoprecipitation of cell lysates. This is the first report of a stable prion infection in muscle cells in vitro and of a long-term prion infection in a nondividing, differentiated peripheral cell type in culture. These in vitro studies also suggest that in vivo prion infection of skeletal muscle requires contact with prion-infected neurons or, possibly, nerve terminals.

Prion infection of muscle cells in vitro. Publishing Authors By Initials

WM Dlakic,E Grigg,RA Bessen,

For similar proteins: prions research abstracts see: proteins: prions research

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Prion infection of muscle cells in vitro. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of virology

VOLUME: 81

Page Numbers: 4615-24

Journal Abbreviation:

ISSN: 0022-538X

DAY: 21

MONTH: 02

YEAR: 2007

Prion infection of muscle cells in vitro. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Prion infection of muscle cells in vitro. Keywords Mesh Terms:

KEYWORDS: Prions

MESH TERMS: metabolism

Chemical & Substance for Abstract: Prion infection of muscle cells in vitro. Information

Substance Name: Prions

Registry Number: 0

Grant and Affiliation Information for Prion infection of muscle cells in vitro.

AFFILIATION: Department of Veterinary Molecular Biology, Montana State University, P.O. Box 173610, Bozeman, MT 59717, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI 055043

ACRONYM: AI

MEDLINETA: J Virol

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