Primitive hematopoietic cell populations reside in the spleen: Studies in the pig, baboon, and human.

Primitive hematopoietic cell populations reside in the spleen: Studies in the pig, baboon, and human. Research Abstract Details 

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Primitive hematopoietic cell populations reside in the spleen: Studies in the pig, baboon, and human. Abstract Text:

Frank J M F Dor,Mario L Ramirez,Kalindi Parmar,Erica L Altman,Christene A Huang,Julian D Down,David K C Cooper,Frank J M F Dor,Mario L Ramirez,Kalindi Parmar,Erica L Altman,Christene A Huang,Julian D Down,David K C Cooper,

OBJECTIVE: We previously observed high levels (>40%) of multilineage hematopoietic cell chimerism following spleen transplantation across full MHC barriers in immunosuppressed miniature swine. We therefore investigated the spleen as a source of hematopoietic progenitor cells (HPCs). MATERIALS AND METHODS: Specific cell-surface markers were used to identify HPCs in the spleen and bone marrow (BM) of young adult (n = 15) and fetal (n = 9) miniature swine by flow cytometry. Hoechst dye-effluxing side population (SP) cells were analyzed in adult spleen, BM, and blood for their expression of c-kit. Functional HPC activity of varying repopulation potential in vitro was investigated by the ability of spleens and BM to give rise to colony-forming units (CFUs) and cobblestone area-forming cells (CAFCs) in long-term stromal cultures. Studies were also carried out on baboon and human spleens and BM. RESULTS: Spleen c-kit+ cells co-expressed more lymphoid markers, but equal myeloid markers, when compared with BM c-kit+ cells. BM and spleen both contained significant percentages of c-kit+ SP cells. Although the frequency of early-forming CFUs in the spleen was only 0.1 to 1.3% of that in the BM, the frequency of CAFCs developing after 8 weeks in culture was comparable to that of BM. Secondary CFUs in long-term culture-initiating cell assays confirmed the presence of long-term repopulating cells at comparable frequencies in spleen and BM. Similar findings were found with regard to baboon and human spleen cells. CONCLUSION: The adult spleen is a relatively rich source of very primitive HPCs, possibly hematopoietic stem cells (HSCs), and may be of therapeutic value.

Primitive hematopoietic cell populations reside in the spleen: Studies in the pig, baboon, and human. Publishing Authors By Initials

FJ Dor,ML Ramirez,K Parmar,EL Altman,CA Huang,JD Down,DK Cooper,FJ Dor,ML Ramirez,K Parmar,EL Altman,CA Huang,JD Down,DK Cooper,

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Primitive hematopoietic cell populations reside in the spleen: Studies in the pig, baboon, and human. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental hematology

VOLUME: 34

Page Numbers: 1573-82

Journal Abbreviation: Exp. Hematol.

ISSN: 0301-472X

DAY: 18

MONTH: Nov

YEAR: 2006

Primitive hematopoietic cell populations reside in the spleen: Studies in the pig, baboon, and human. Information

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LANGUAGE: eng

NlmUniqueID: 402313

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Grant and Affiliation Information for Primitive hematopoietic cell populations reside in the spleen: Studies in the pig, baboon, and human.

AFFILIATION: Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Mass., USA.

Country: Netherlands

AGENCY: United States PHS

GRANT: 1P01 A145897

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MEDLINETA: Exp Hematol

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