Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus).

Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). Research Abstract Details 

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Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). Abstract Text:

Y Okamoto,K Ogawa,T Motohiro,N Nishi,E Muta,S Ota,

Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombrine I gave three bands upon PAGE. Scombrine II (scombrine gamma) consists of 34 amino acid residues, and its sequence is: Pro-Arg-Arg-Arg-Arg-Arg-Ala-Ser-Arg-Pro-Val-Arg-Arg-Arg-Arg-Arg- Ala-Arg-Arg-Ser-Thr-Ala-Val-Arg-Arg-Arg-Arg-Arg-Val-Val-Arg-Arg-Arg-Arg. The ion spray mass spectrum shows that scombrine gamma has a molecular mass of 4,532.13 Da. The other minor component (scombrine I) is considered to be a mixture of degradation products of scombrine gamma based on the results of PAGE and ion spray mass spectrometry. Scombrine gamma has a similar sequence to sardaine Z2 from striped bonito except for two positions.

Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). Publishing Authors By Initials

Y Okamoto,K Ogawa,T Motohiro,N Nishi,E Muta,S Ota,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

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Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 113

Page Numbers: 658-64

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1993

Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). Information

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LANGUAGE: eng

NlmUniqueID: 376600

Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus). Information

Substance Name: Pepsin A

Registry Number: EC 3.4.23.1

Grant and Affiliation Information for Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus).

AFFILIATION: Department of Biochemistry, Daiichi College of Pharmaceutical Sciences, Fukuoka.

Country: JAPAN

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MEDLINETA: J Biochem

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