Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia.

Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia. Research Abstract Details 

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Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia. Abstract Text:

F Fukumori,N Takeuchi,T Hagiwara,H Ohbayashi,T Endo,N Kochibe,Y Nagata,A Kobata,

Aleuria aurantia lectin (AAL) is a protein composed of two identical subunits having no carbohydrate chain and shows sugar-binding specificity for L-fucose. Full-length cDNA encoding for the lectin has been isolated from a lambda gt11 library, screened with an antiserum directed against AAL. The cDNA clone contained 1,370 nucleotides and an open reading frame of 939 nucleotides encoding 313 amino acids. The amino-terminal sequence (residues 1-30) of the lectin isolated from the mushroom coincided with the deduced amino acid sequence starting from proline at the 2nd residue, indicating that the mature AAL consists of 312 amino acids. Its molecular weight is calculated to be 33,398. The deduced amino acid sequence shows that AAL includes six internal homologous regions, and has considerable homology with a hemagglutinin from a Gram-negative bacterium, Myxococcus xanthus, which forms a fruiting body. No significant homology was observed with higher plant or animal lectins. The recombinant AAL produced by Escherichia coli JM109 carrying the AAL expression plasmid pKA-1 [Fukumori, F. et al. (1989) FEBS Lett. 250, 153-156] was purified from the cell lysate by affinity chromatography using a fucose-starch column, and hundreds of milligrams of the lectin was obtained. The recombinant lectin showed the same biochemical characteristics and sugar binding specificity as did the natural AAL.

Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia. Publishing Authors By Initials

F Fukumori,N Takeuchi,T Hagiwara,H Ohbayashi,T Endo,N Kochibe,Y Nagata,A Kobata,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, nucleic acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, nucleic acid research

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Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 107

Page Numbers: 190-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1990

Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Nucleic Acid

MESH TERMS: metabolism

Chemical & Substance for Abstract: Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Primary structure of a fucose-specific lectin obtained from a mushroom, Aleuria aurantia.

AFFILIATION: Research Institute, Nichirei Corporation, Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: D00573

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