Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi.

Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi. Research Abstract Details 

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Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi. Abstract Text:

H Watanabe,A Naitoh,Y Suyama,N Inokuchi,H Shimada,T Koyama,K Ohgi,M Irie,

The complete primary structure of a base non-specific and adenylic acid preferential RNase (RNase M) from Aspergillus saitoi was determined. The sequence was determined by analysis of the peptides generated by digestion of heat-denatured RNase M with lysylendopeptidase, and the peptides generated from RCM RNase M by digestion with staphylococcal V8 protease or chemical cleavage with BrCN. It consisted of 238 amino acid residues and carbohydrate moiety attached to the 74th asparagine residue. The molecular weight of the protein moiety deduced from the sequence was 26,596. The locations of 10 half cystine residues are almost superimposable on those of RNase Rh from Rhizopus niveus and RNase T2 from Aspergillus oryzae which have similar base specificity. The homology between RNase M and RNase Rh and RNase T2 amounted to 97 and 160 amino acid residues, respectively. The amino acid sequences conserved in the three RNases are concentrated around the three histidine residues, which are supposed to form part of the active sites of these RNases.

Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi. Publishing Authors By Initials

H Watanabe,A Naitoh,Y Suyama,N Inokuchi,H Shimada,T Koyama,K Ohgi,M Irie,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tryptophan research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tryptophan research

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Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 303-10

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1990

Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi. Keywords Mesh Terms:

KEYWORDS: Tryptophan

MESH TERMS: analysis

Chemical & Substance for Abstract: Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi. Information

Substance Name: Endopeptidases

Registry Number: EC 3.4.-

Grant and Affiliation Information for Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi.

AFFILIATION: Department of Microbiology, Hoshi College of Pharmacy, Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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