Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro.

Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro. Research Abstract Details 

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Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro. Abstract Text:

Shanley Blackley,Zhihua Kou,Huiyuan Chen,Matthew Quinn,Robert C Rose,Jacob J Schlesinger,Myra Coppage,Xia Jin,Shanley Blackley,Zhihua Kou,Huiyuan Chen,Matthew Quinn,Robert C Rose,Jacob J Schlesinger,Myra Coppage,Xia Jin,

Understanding the pathogenesis of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) requires the precise identification of dengue virus (DV)-permissive target cells. In a previous study using unfractionated human peripheral blood mononuclear cells, we found that monocytes, but not B or T cells, were the principal DV-permissive cells in the absence of DV-immune pooled human sera (PHS) and the major mediators of antibody-dependent enhancement in the presence of PHS. To further identify DV-permissive target cells in other tissues and organs, we isolated human splenic mononuclear cells (MNCs), inoculated them with DV type 2 (strain 16681) in the presence or absence of PHS, and assessed their infection either directly using flow cytometry and reverse transcription-PCR (RT-PCR) assays or indirectly by plaque assay. We found that in the absence of PHS, a small proportion of splenic macrophages appeared to be positive for DV antigens in comparison to staining controls by the flow cytometric assay (0.77% +/- 1.00% versus 0.18% +/- 0.12%; P = 0.07) and that viral RNA was detectable by the RT-PCR assay in MNCs exposed to DV. Additionally, supernatants from cultures of DV-exposed MNCs contained infectious virions that were readily detectable by plaque assay. The magnitude of infection was significantly enhanced in splenic macrophages in the presence of highly diluted PHS (5.41% +/- 3.53% versus 0.77% +/- 1.00%; P = 0.001). In contrast, primary T and B cells were not infected in either the presence or absence of PHS. These results provide evidence, for the first time, that human primary splenic macrophages, rather than B or T cells, are the principal DV-permissive cells in the spleen and that they may be uniquely important in the initial steps of immune enhancement that leads to DHF/DSS in some DV-infected individuals.

Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro. Publishing Authors By Initials

S Blackley,Z Kou,H Chen,M Quinn,RC Rose,JJ Schlesinger,M Coppage,X Jin,S Blackley,Z Kou,H Chen,M Quinn,RC Rose,JJ Schlesinger,M Coppage,X Jin,

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Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of virology

VOLUME: 81

Page Numbers: 13325-34

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 10

MONTH: 10

YEAR: 2007

Primary human splenic macrophages, but not T or B cells, are the principal target cells for dengue virus infection in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 113724

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AFFILIATION: Department of Biology, University of Rochester, Rochester, New York 14642, USA.

Country: United States

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MEDLINETA: J Virol

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