Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene.

Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene. Research Abstract Details 

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Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene. Abstract Text:

Shuji Miyagawa,Shino Nakatsu,Takatoshi Nakagawa,Akihiro Kondo,Katsuyoshi Matsunami,Kenji Hazama,Junko Yamada,Keizo Tomonaga,Takayuki Miyazawa,Ryota Shirakura,

The possibility of preventing the transmission of porcine endogenous retrovirus (PERV) to human cells using short interfering RNAs (siRNA) was investigated. The siRNA for the p30 of PERV gag region was cloned into pSUPER, the polymerase-III H1-RNA gene promoter. A green fluorescence protein (GFP) was also cloned into pSUPER to establish pSXGH. Pig endothelial cells (PEC) were transduced with the LacZ gene by pseudotype infection, and infected with PERV subtype B, resulting in the formation of PEC(LacZ)/PB. The PEC(LacZ)/PB was next transfected with pSXGH-siRNA. The expression of siRNA was provisionally checked by determining the level of expression of GFP. Culture supernatants of infected cells were then inoculated into HEK293 cells. The siRNA clearly destroyed the PERV infectivity of PEC(LacZ)/PB in both transient cell lines and stable clones. Moreover, the decreased levels of mRNA and gag protein were evidenced in the stable clones by real-time PCR and Western blotting, respectively. The final goal of our study was to establish a transgenic pig expressing the siRNA for PERV. The results suggest that siRNA represents a novel approach for controlling PERV infections in clinical xenotransplantation.

Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene. Publishing Authors By Initials

S Miyagawa,S Nakatsu,T Nakagawa,A Kondo,K Matsunami,K Hazama,J Yamada,K Tomonaga,T Miyazawa,R Shirakura,

For similar surgical procedures, operative: transplantation: transplantation, heterologous research abstracts see: surgical procedures, operative: transplantation: transplantation, heterologous research

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Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 137

Page Numbers: 503-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 2005

Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene. Keywords Mesh Terms:

KEYWORDS: Transplantation, Heterologous

MESH TERMS: adverse effects

Chemical & Substance for Abstract: Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene. Information

Substance Name: RNA, Small Interfering

Registry Number: 0

Grant and Affiliation Information for Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene.

AFFILIATION: Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871. miyagawa@orgtrp.med.osaka-u.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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