Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa.

Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa. Research Abstract Details 

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Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa. Abstract Text:

D K Augustin,Y Song,M S Baek,Y Sawa,G Singh,B Taylor,A Rubio-Mills,J L Flanagan,J P Wiener-Kronish,S V Lynch,

Pseudomonas aeruginosa is one of the major causative agents of mortality and morbidity in hospitalized patients due to a multiplicity of virulence factors associated with both chronic and acute infections. Acute P. aeruginosa infection is primarily mediated by planktonic bacteria expressing the type III secretion system (TTSS), a surface-attached needle-like complex that injects cytotoxins directly into eukaryotic cells, causing cellular damage. Lipopolysaccharide (LPS) is the principal surface-associated virulence factor of P. aeruginosa. This molecule is known to undergo structural modification (primarily alterations in the A- and B-band O antigen) in response to changes in the mode of life (e.g., from biofilm to planktonic). Given that LPS exhibits structural plasticity, we hypothesized that the presence of LPS lacking O antigen would facilitate eukaryotic intoxication and that a correlation between the LPS O-antigen serotype and TTSS-mediated cytotoxicity would exist. Therefore, strain PAO1 (A+ B+ O-antigen serotype) and isogenic mutants with specific O-antigen defects (A+ B-, A- B+, and A- B-) were examined for TTSS expression and cytotoxicity. A strong association existed in vitro between the absence of the large, structured B-band O antigen and increased cytotoxicity of these strains. In vivo, all three LPS mutant strains demonstrated significantly increased lung injury compared to PAO1. Clinical strains lacking the B-band O antigen also demonstrated increased TTSS secretion. These results suggest the existence of a cooperative association between LPS O-antigen structure and the TTSS in both laboratory and clinical isolates of P. aeruginosa.

Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa. Publishing Authors By Initials

DK Augustin,Y Song,MS Baek,Y Sawa,G Singh,B Taylor,A Rubio-Mills,JL Flanagan,JP Wiener-Kronish,SV Lynch,

For similar biological factors: toxins, biological: virulence factors research abstracts see: biological factors: toxins, biological: virulence factors research

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Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 2203-9

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 5

MONTH: 01

YEAR: 2007

Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa. Keywords Mesh Terms:

KEYWORDS: Virulence Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa. Information

Substance Name: Virulence Factors

Registry Number: 0

Grant and Affiliation Information for Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa.

AFFILIATION: Biology Department, California State University, San Francisco, CA 94142, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: PH50HL74005

ACRONYM: HL

MEDLINETA: J Bacteriol

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