Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.).

Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.). Research Abstract Details 

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Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.). Abstract Text:

Yuxin Chen,Colin T Mant,Robert S Hodges,

The present study examines the effect of reversed-phase high-performance liquid chromatography (RP-HPLC) column diameter (1mm to 9.4mm I.D.) on the one-step slow gradient preparative purification of a 26-residue synthetic antimicrobial peptide. When taken together, the semi-preparative column (9.4mm I.D.) provided the highest yields of purified product (an average of 90.7% recovery from hydrophilic and hydrophobic impurities) over a wide range of sample load (0.75-200mg). Columns with smaller diameters, such as narrowbore columns (150x2.1mm I.D.) and microbore columns (150x1.0mm I.D.), can be employed to purify peptides with reasonable recovery of purified product but the range of the crude peptide that can be applied to the column is limited. In addition, the smaller diameter columns require more extensive fraction analysis to locate the fractions of pure product than the larger diameter column with the same load. Our results show the excellent potential of the one-step slow gradient preparative protocol as a universal method for purification of synthetic peptides.

Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.). Publishing Authors By Initials

Y Chen,CT Mant,RS Hodges,

For similar investigative techniques: chemistry, analytical: chromatography: chromatography, liquid: chromatography, high pressure liquid research abstracts see: investigative techniques: chemistry, analytical: chromatography: chromatography, liquid: chromatography, high pressure liquid research

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Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.). Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of chromatography. A

VOLUME: 1140

Page Numbers: 112-20

Journal Abbreviation:

ISSN: 0021-9673

DAY: 6

MONTH: 12

YEAR: 2006

Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9318488

Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.). Keywords Mesh Terms:

KEYWORDS: Chromatography, High Pressure Liquid

MESH TERMS: methods

Chemical & Substance for Abstract: Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.). Information

Substance Name: D-V13K(D) peptide

Registry Number: 0

Grant and Affiliation Information for Preparative reversed-phase high-performance liquid chromatography collection efficiency for an antimicrobial peptide on columns of varying diameters (1mm to 9.4mm I.D.).

AFFILIATION: Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA.

Country: Netherlands

AGENCY: United States NIGMS

GRANT: R01 GM61855

ACRONYM: GM

MEDLINETA: J Chromatogr A

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