Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.

Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Abstract Text:

Hongwei Xie,Sricharan Bandhakavi,Mikel R Roe,Timothy J Griffin,

Protein sequence database searching of tandem mass spectrometry data is commonly employed to identify post-translational modifications (PTMs) to peptides in global proteomic studies. In these studies, the accurate identification of these modified peptides relies on strategies to ensure high-confidence results from sequence database searching in which differential mass shift parameters are employed to identify PTMs to specific amino acids. Using lysine acetylation as an example PTM, we have observed that the inclusion of differential modification information in sequence database searching dramatically increases the potential for false-positive sequence matches to modified peptides, making the confident identification of true sequence matches difficult. In a proof-of-principle study of whole cell yeast lysates, we demonstrate the combination of preparative isoelectric focusing using free-flow electrophoresis, and an adjusted peptide isoelectric point prediction algorithm, as an effective means to increase the confidence of lysine-acetylated peptide identification. These results demonstrate the potential utility of this general strategy for improving the identification of PTMs which cause a shift to the intrinsic isoelectric point of peptides.

Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Publishing Authors By Initials

H Xie,S Bandhakavi,MR Roe,TJ Griffin,

For similar proteins: fungal proteins: saccharomyces cerevisiae proteins research abstracts see: proteins: fungal proteins: saccharomyces cerevisiae proteins research

PUBMED ID PMID:

MEDLINE DATE:

Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of proteome research

VOLUME: 6

Page Numbers: 2019-26

Journal Abbreviation:

ISSN: 1535-3893

DAY: 31

MONTH: 03

YEAR: 2007

Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101128775

Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Information

Substance Name: Lysine

Registry Number: 56-87-1

Grant and Affiliation Information for Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.

AFFILIATION: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK073731

ACRONYM: DK

MEDLINETA: J Proteome Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures Related Publications

• A field-sweep/field-lock system for superconducting magnets--Application to high-field EPR.
• Acid sphingomyelinase deficiency increases susceptibility to fatal alphavirus encephalomyelitis.
• Commercial aspects of bovine embryo transfer.
• Comparison of low versus high tacrolimus levels in kidney transplantation: assessment of efficacy by protocol biopsies.
• Description of depolarization effects in double-quantum solid state nuclear magnetic resonance experiments using multipole-multimode Floquet theory.
• Dynamic nuclear polarization of amyloidogenic peptide nanocrystals: GNNQQNY, a core segment of the yeast prion protein Sup35p.
• Effects of PKC412, nilotinib, and imatinib against GIST-associated PDGFRA mutants with differential imatinib sensitivity.
• Equine fetal kinetics: Presentation and location.
• Eye movement, vection, and motion sickness with foveal and peripheral vision.
• Hemagglutinin protein is a primary target of the measles virus-specific HLA-A2-restricted CD8+ T cell response during measles and after vaccination.
• High-density lipoprotein and the risk of recurrent venous thromboembolism.
• High-frequency dynamic nuclear polarization using mixtures of TEMPO and trityl radicals.
• Identification of carbonylated proteins from enriched rat skeletal muscle mitochondria using affinity chromatography-stable isotope labeling and tandem mass spectrometry.
• In situ temperature jump high-frequency dynamic nuclear polarization experiments: enhanced sensitivity in liquid-state NMR spectroscopy.
• Incidence of invasive pneumococcal disease among individuals with sickle cell disease before and after the introduction of the pneumococcal conjugate vaccine.
• Increasing Stimulation Frequency Prolongs Force Output in Healthy Thenar Muscle: 1923: Board #211 10:30 AM- 10:30 AM.
• Interpreting treatment effects when cases are institutionalized after treatment.
• Intrinsic stability and functional properties of disulfide bond-stabilized coagulation factor VIIIa variants.
• Multipole-multimode Floquet theory of rotational resonance width experiments: 13C-13C distance measurements in uniformly labeled solids.
• Natural outcome and ultrasonic identification of equine fetal twins.
• Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
• Proteomic mapping of 4-hydroxynonenal protein modification sites by solid-phase hydrazide chemistry and mass spectrometry.
• Proton assisted insensitive nuclei cross polarization.
• Recovery and transfer of equine embryos.
• Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury.
• Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide.
• Spontaneously reduced blood pressure load in the rat streptozotocin-induced diabetes model: potential pathogenetic relevance.
• Sustaining knowledge transfer through leadership.
• Synergistic roles of antibody and interferon in noncytolytic clearance of Sindbis virus from different regions of the central nervous system.
• TOTAPOL: a biradical polarizing agent for dynamic nuclear polarization experiments in aqueous media.