Preparation of the membrane-permeant biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins.

Preparation of the membrane-permeant biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins. Research Abstract Details 

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Preparation of the membrane-permeant biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins. Abstract Text:

The membrane-permeant fluorogenic biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin, respectively. Handling of toxic reagents such as arsenic trichloride is minimized so the synthesis can be carried out in a typical chemistry laboratory, usually taking about 2-3 d. A wide range of other biarsenical reagents and intermediates that also bind to tetracysteine-tagged (CysCysProGlyCysCys) proteins can be prepared similarly using this general procedure.

Preparation of the membrane-permeant biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins. Publishing Authors By Initials

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Preparation of the membrane-permeant biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nature protocols

VOLUME: 3

Page Numbers: 1527-34

Journal Abbreviation: Nat Protoc

ISSN: 1750-2799

DAY: 5

MONTH: 09

YEAR: 2008

Preparation of the membrane-permeant biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins. Information

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LANGUAGE: eng

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AFFILIATION: Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0647, USA.

Country: England

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