Preparation of protein extracts from yeast.

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Preparation of protein extracts from yeast. Abstract Text:

B Dunn,C R Wobbe,B Dunn,C R Wobbe,

Three protocols are presented for preparing protein extracts; they differ primarily in the way the cells are broken. In the basic protocol, cells are enzymatically converted to spheroplasts, which are then lysed by a combination of osmotic shock and Dounce homogenization. A support protocol for isolating intact nuclei by differential centrifugation is also presented. An alternate protocol describes mechanical breakage of cells by vortexing in the presence of glass beads. In a second alternate protocol, growing cells are frozen immediately in liquid nitrogen and then lysed by grinding in an industrial-strength blender in the presence of liquid nitrogen.

Preparation of protein extracts from yeast. Publishing Authors By Initials

B Dunn,CR Wobbe,B Dunn,CR Wobbe,

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Preparation of protein extracts from yeast. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in molecular biology / edited by

VOLUME: Chapter 13

Page Numbers: Unit13.13

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ISSN: 1934-3647

DAY: 11

MONTH: May

YEAR: 2001

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LANGUAGE: eng

NlmUniqueID: 8908160

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AFFILIATION: Memorial Sloan-Kettering Cancer Center, New York, New York.

Country: United States

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MEDLINETA: Curr Protoc Mol Biol

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